Flores A, Gastélum E M, Bosseno M F, Ordoñez R, Kasten F L, Espinoza B, Ramsey J, Brenière S F
UNAM, Instituto de Investigaciones Biomédicas, AP 70228, CP 04510, D.F., México, Mexico.
Infect Genet Evol. 2001 Jul;1(1):21-8. doi: 10.1016/s1567-1348(01)00005-3.
Triatoma barberi, T. dimidiata, T. longipennis, T. pallidipennis and T. picturata, all key Chagas disease vectors in Mexico, were analysed by multilocus enzyme electrophoresis (MLEE) at 17 putative loci. The majority of insect specimens studied were collected from domestic and peridomestic structures from multiple geographic locations while others were collected from sylvatic areas. T. barberi was the least polymorphic species (P(0.95)=0.18), with polymorphism rates of the other species ranging from 0.29 to 0.50. T. barberi, a member of the protracta complex, clustered apart from the other studied species by Nei's genetic distance with >1.36, and at least eight loci were found to be diagnostic for this species. T. dimidiata was more related to T. longipennis, T. pallidipennis and T. picturata (phyllosoma complex) than to T. barberi, with a genetic distance averaging 0.36 with the phyllosoma complex species. In contrast, the genetic distances between the three phyllosoma complex species were not significantly different from zero, and there were no species-specific loci differentiating among them. The results strongly support the grouping of these three species in one complex, separate from the two other species studied.
巴氏锥猎蝽、二色锥猎蝽、长翅锥猎蝽、淡色锥猎蝽和花斑锥猎蝽都是墨西哥恰加斯病的主要传播媒介，对它们在17个假定基因座上进行了多位点酶电泳（MLEE）分析。大多数研究的昆虫标本是从多个地理位置的家庭和家庭周边建筑中采集的，其他标本则是从野生环境中采集的。巴氏锥猎蝽是多态性最低的物种（P(0.95)=0.18），其他物种的多态性率在0.29至0.50之间。巴氏锥猎蝽是protracta复合体的成员，通过内氏遗传距离与其他研究物种聚类分开，遗传距离大于1.36，并且发现至少有8个基因座对该物种具有诊断性。二色锥猎蝽与长翅锥猎蝽、淡色锥猎蝽和花斑锥猎蝽（叶体复合体）的关系比与巴氏锥猎蝽更密切，与叶体复合体物种的遗传距离平均为0.36。相比之下，叶体复合体的三个物种之间的遗传距离与零没有显著差异，并且没有物种特异性基因座来区分它们。结果有力地支持了将这三个物种归为一个复合体，与其他两个研究物种分开。
