Sherratt T G, Vulliamy T, Strong P N
Department of Paediatrics and Neonatal Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Biochem J. 1992 Nov 1;287 ( Pt 3)(Pt 3):755-9. doi: 10.1042/bj2870755.
Dystrophin cDNA fragments encoding the C-terminal repeats of the central rod region have been expressed as fusion proteins. The polyclonal antisera raised to the purified fusion proteins have been characterized and neither antiserum cross-reacted with dystrophin-related protein. Antisera detected dystrophin with molecular mass close to that of the human in all terrestrial vertebrates and amphibia studied. Experiments with antisera to the N-terminal region of the dystrophin rod confirmed that epitopes to the rod region were conserved during this evolutionary period and the length of this domain remained unaltered.
编码中央杆状区域C末端重复序列的肌营养不良蛋白cDNA片段已被表达为融合蛋白。针对纯化融合蛋白产生的多克隆抗血清已得到鉴定，且两种抗血清均未与肌营养不良蛋白相关蛋白发生交叉反应。在所研究的所有陆生脊椎动物和两栖动物中，抗血清检测到的肌营养不良蛋白分子量与人的接近。用针对肌营养不良蛋白杆状结构N末端区域的抗血清进行的实验证实，在这个进化时期，杆状区域的表位是保守的，并且该结构域的长度保持不变。
