Kim H, Arakawa Hideo, Osada Toshiya, Ikai Atsushi
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):359-63. doi: 10.1016/S0304-3991(03)00061-5.
Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy (AFM). First, the distribution of the integrin beta(5) subunit which constitutes a part of the VN receptor on the cell was confirmed by conventional immunohistochemistry after fixing the cell. To visualize the distribution of the receptor on a living cell by an independent and potentially a more quantitative method, the AFM was used with a microbead attached to the cantilever tip to increase the area of contact and VN was immobilized on the microbead. Force measurements were then performed over a large area of a living murine osteoblastic cell using the microbead covered with VN.
利用原子力显微镜（AFM）成功测定了活体小鼠成骨细胞上玻连蛋白（VN）受体的分布。首先，在固定细胞后，通过传统免疫组织化学方法确认了构成细胞上VN受体一部分的整合素β(5)亚基的分布。为了通过一种独立且可能更具定量性的方法可视化活细胞上受体的分布，将附着有微珠的AFM用于增加接触面积，并将VN固定在微珠上。然后使用覆盖有VN的微珠在大面积的活体小鼠成骨细胞上进行力测量。
