Mora Marirosa, Veggi Daniele, Santini Laura, Pizza Mariagrazia, Rappuoli Rino
IRIS, Chiron S.r.l., via Fiorentina 1, 53100 Siena, Italy.
Drug Discov Today. 2003 May 15;8(10):459-64. doi: 10.1016/s1359-6446(03)02689-8.
Whole-genome sequencing of bacteria and advances in bioinformatics have revolutionized the vaccinology field, leading to the identification of potential vaccine candidates without the need for cultivating the pathogen. This approach, termed "reverse vaccinology", reduces the time and cost required for the identification of candidate vaccines and provides new solutions for those diseases for which conventional approaches have failed. The first example of the potential of reverse vaccinology has been the identification of novel antigens of meningococcus B as potential candidates for a novel and effective vaccine. The same approach has been successfully applied to other important human pathogens, demonstrating the feasibility to develop vaccines against any infectious disease. This review focuses on some recent advances in the identification of vaccine candidates by mining the genomic sequences of pathogenic bacteria.
细菌的全基因组测序和生物信息学的进展彻底改变了疫苗学领域，无需培养病原体就能鉴定出潜在的疫苗候选物。这种方法被称为“反向疫苗学”，减少了鉴定候选疫苗所需的时间和成本，并为传统方法失败的疾病提供了新的解决方案。反向疫苗学潜力的第一个例子是鉴定出B型脑膜炎球菌的新型抗原作为新型有效疫苗的潜在候选物。同样的方法已成功应用于其他重要的人类病原体，证明了开发针对任何传染病疫苗的可行性。本综述重点介绍了通过挖掘病原菌基因组序列鉴定疫苗候选物的一些最新进展。
