Modelling the individual cell lag phase. Isolating single cells: protocol development.
作者信息
Francois K, Devlieghere F, Standaert A R, Geeraerd A H, Van Impe J F, Debevere J
机构信息
Laboratory of Food Microbiology and Preservation, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
出版信息
Lett Appl Microbiol. 2003;37(1):26-30. doi: 10.1046/j.1472-765x.2003.01340.x.
AIMS
To develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield.
METHODS AND RESULTS
Single cells were obtained using 1/2 dilution series in microtitre plates. Seventy-two Lactococcus lactis dilution series were checked by plate counting. When the last five columns of the plates were observed, the chance of having one single cell was 80%, while the yield was 75 wells containing cells. A simulation model confirmed these results. This method was compared with the commonly applied method.
CONCLUSIONS
This method makes it possible to combine a higher chance of having one cell in a microtitre well with a slightly higher yield.
SIGNIFICANCE AND IMPACT OF THE STUDY
A tool is developed to isolate single cells to provide a suitable base for investigating and modelling the individual cell lag phase.
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