边缘无形体msp5基因编码一种在所有已识别的无形体物种中保守的19千道尔顿蛋白。
The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species.
作者信息
Visser E S, McGuire T C, Palmer G H, Davis W C, Shkap V, Pipano E, Knowles D P
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
出版信息
Infect Immun. 1992 Dec;60(12):5139-44. doi: 10.1128/iai.60.12.5139-5144.1992.
Immunization with Anaplasma marginale outer membranes induced immunity against clinical disease which correlated with antibody titer to outer membrane proteins, including a 19-kDa protein (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). This 19-kDa protein, designated major surface protein 5 (MSP-5), was encoded by a single-copy 633-bp gene. The molecular mass of MSP-5, defined in immunoblots by binding to monoclonal antibody ANAF16C1, was conserved among all recognized species of Anaplasma: A. marginale, A. centrale, and A. ovis. Recombinant MSP-5, which absorbed the antibody reactivity of bovine immune serum to native MSP-5, was recognized by anti-A. marginale and anti-A. centrale immune sera in a competitive inhibition assay with monoclonal antibody ANAF16C1. The presence of antibody to the epitope defined by monoclonal antibody ANAF16C1 in all postinfection sera tested indicates that this epitope is a potential diagnostic antigen for use in identifying persistently infected cattle.
用边缘无形体外膜进行免疫接种可诱导针对临床疾病的免疫力，这种免疫力与针对外膜蛋白（包括一种19-kDa蛋白）的抗体滴度相关（N. 特贝勒、T. C. 麦圭尔和G. H. 帕尔默，《感染与免疫》59:3199 - 3204，1991年）。这种19-kDa蛋白被命名为主要表面蛋白5（MSP-5），由一个单拷贝的633-bp基因编码。通过与单克隆抗体ANAF16C1结合在免疫印迹中确定的MSP-5的分子量，在边缘无形体、中央无形体和绵羊无形体所有已确认的种中是保守的。重组MSP-5吸收了牛免疫血清对天然MSP-5的抗体反应性，在与单克隆抗体ANAF16C1的竞争性抑制试验中被边缘无形体和中央无形体免疫血清识别。在所有检测的感染后血清中存在针对单克隆抗体ANAF16C1所定义表位的抗体，这表明该表位是用于识别持续感染牛的潜在诊断抗原。
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