McGuire T C, Perryman L E, Davis W C
Am J Vet Res. 1983 Jul;44(7):1284-8.
Nine monoclonal antibodies which reacted with equine immunoglobulin (Ig)M and not other equine Ig and serum proteins were prepared. Cells producing antibodies (C 1.9) which precipitated with IgM and bound to staphylococcal protein A were triple-cloned (C 1.9/3.2) and the antibodies further characterized. Monoclonal antibody C 1.9/3.2 reacted with an IgM determinant present on serum IgM from horses of several breeds. Studies with 125I-labeled IgM revealed the presence of this determinant on all IgM molecules. The monoclonal antibody enabled quantitation of IgM in presuckling foal and adult horse sera, using rocket electrophoresis. This procedure was used because presumably it gives a positive precipitation reaction over a wide range of antigen-antibody ratios. The C 1.9/3.2 monoclonal antibody recognized an exposed mu-chain determinant on live B lymphocytes, as determined by immunofluorescence. Also, IgM-containing cells could be identified in acetone-fixed frozen sections of lymphoid tissue. Sera from several other species carry the determinant identified by C 1.9/3.2, suggesting that the reagent may be useful for IgM studies in other species.
制备了9种单克隆抗体，它们与马免疫球蛋白（Ig）M反应，而不与其他马Ig和血清蛋白反应。产生与IgM沉淀并与葡萄球菌蛋白A结合的抗体（C 1.9）的细胞进行了三次克隆（C 1.9/3.2），并对抗体进行了进一步表征。单克隆抗体C 1.9/3.2与几个品种马的血清IgM上存在的IgM决定簇反应。对125I标记的IgM的研究表明，所有IgM分子上都存在这个决定簇。该单克隆抗体能够使用火箭电泳法定量测定哺乳前驹和成年马血清中的IgM。使用该方法是因为据推测它在广泛的抗原-抗体比例范围内都能产生阳性沉淀反应。通过免疫荧光测定，C 1.9/3.2单克隆抗体识别活B淋巴细胞上暴露的μ链决定簇。此外，在淋巴组织的丙酮固定冰冻切片中可以识别出含IgM的细胞。其他几个物种的血清带有C 1.9/3.2识别的决定簇，这表明该试剂可能对其他物种的IgM研究有用。
