Khan M I, Fadl A A, Venkitanarayanan K S
Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA.
J Appl Microbiol. 2003;95(1):142-5. doi: 10.1046/j.1365-2672.2003.01953.x.
To evaluate the ability of Salmonella enterica ser. Enteritidis outer membrane proteins (OMPs) of 75.6 and 82.3 kDa to inhibit or reduce in vivo colonization of S. Enteritidis on intestinal mucosa in chickens.
Nine-week-old specific-pathogen-free chickens were subcutaneously immunized with 75.6 or 82.3 kDa protein, and challenged with a virulent strain of S. Enteritidis. Chickens were killed, and portions of small intestine and caecum were removed at necropsy. The population of S. Enteritidis attached to chicken intestinal mucosa was determined. The population of S. Enteritidis recovered from the small intestine and caecum of chickens immunized with 75.6 or 82.3 kDa protein was significantly (P < 0.05) lower than that recovered from the control birds.
Salmonella Enteritidis OMPs 75.6 kDa and 82.3 kDa were effective in reducing colonization of S. Enteritidis on intestinal mucosa in chickens.
Salmonella Enteritidis OMPs 75.6 or 82.3 kDa could be used as potential vaccines to reduce S. Enteritidis colonization in chickens.
评估肠炎沙门氏菌肠炎亚种75.6 kDa和82.3 kDa的外膜蛋白（OMPs）抑制或减少肠炎沙门氏菌在鸡肠道黏膜上体内定植的能力。
用75.6 kDa或82.3 kDa蛋白对9周龄无特定病原体鸡进行皮下免疫，然后用肠炎沙门氏菌强毒株进行攻毒。对鸡实施安乐死后，在尸检时取出部分小肠和盲肠。测定附着在鸡肠道黏膜上的肠炎沙门氏菌数量。从用75.6 kDa或82.3 kDa蛋白免疫的鸡的小肠和盲肠中回收的肠炎沙门氏菌数量显著（P<0.05）低于从对照鸡中回收的数量。
75.6 kDa和82.3 kDa的肠炎沙门氏菌外膜蛋白能有效减少肠炎沙门氏菌在鸡肠道黏膜上的定植。
75.6 kDa或82.3 kDa的肠炎沙门氏菌外膜蛋白可用作潜在疫苗，以减少肠炎沙门氏菌在鸡体内的定植。
