Singh-Zocchi Mukta, Dixit Sanhita, Ivanov Vassili, Zocchi Giovanni
Department of Physics and Astronomy, University of California, Los Angeles, CA 90095-1547, USA.
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7605-10. doi: 10.1073/pnas.1337215100. Epub 2003 Jun 13.
We demonstrate the detection of nanometer-scale conformational changes of single DNA oligomers through a micromechanical technique. The quantity monitored is the displacement of a micrometer-size bead tethered to a surface by the probe molecule undergoing the conformational change. This technique allows probing of conformational changes within distances beyond the range of fluorescence resonance energy transfer. We apply the method to detect single hybridization events of label-free target oligomers. Hybridization of the target is detected through the conformational change of the probe.
我们通过一种微机械技术展示了对单个DNA寡聚物纳米级构象变化的检测。所监测的量是通过经历构象变化的探针分子 tethered 到表面的微米级珠子的位移。该技术允许在荧光共振能量转移范围之外的距离内探测构象变化。我们应用该方法检测无标记靶寡聚物的单个杂交事件。通过探针的构象变化检测靶标的杂交。
原文中“tethered”这个词不太完整,可能影响准确理解,但按照要求逐字翻译了。