Oh Myungkeun, Jayasooriya Vidura, Woo Sung Oh, Nawarathna Dharmakeerthi, Choi Yongki
Materials and Nanotechnology Program, North Dakota State University, Fargo, North Dakota 58108, USA.
Department of Electrical and Computer Engineering, North Dakota State University, Fargo, North Dakota 58108, USA.
ACS Appl Nano Mater. 2020 Jan 24;3(1):797-805. doi: 10.1021/acsanm.9b02302. Epub 2020 Jan 3.
Insulator-based dielectrophoretic (iDEP) trapping, separating, and concentrating nanoscale objects is carried out using a non-metal, unbiased, mobile tip acing as a tweezers. The spatial control and manipulation of fluorescently-labeled polystyrene particles and DNA were performed to demonstrate the feasibility of the iDEP tweezers. Frequency-dependent iDEP tweezers' strength and polarity were quantitatively determined using two theoretical approaches to DNA, which resulted in a factor of 2 ~ 40 differences between them. In either approach, the strength of iDEP was at least 4-order of magnitude stronger than the thermal force, indicating iDEP was a dominant force for trapping, holding, and separating DNA. The trapping strength and volume of the iDEP tweezers were also determined, which further supports direct capture and manipulation of DNA at the tip end.
基于绝缘体的介电泳（iDEP）捕获、分离和浓缩纳米级物体是通过使用一个非金属、无偏置、可移动的尖端作为镊子来实现的。对荧光标记的聚苯乙烯颗粒和DNA进行了空间控制和操作，以证明iDEP镊子的可行性。使用两种针对DNA的理论方法定量确定了频率依赖性iDEP镊子的强度和极性，结果它们之间存在2至40倍的差异。在任何一种方法中，iDEP的强度都比热力至少强4个数量级，表明iDEP是捕获、固定和分离DNA的主导力。还确定了iDEP镊子的捕获强度和体积，这进一步支持了在尖端直接捕获和操作DNA。
