Sanz F, Coll J M
Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Arch Virol. 1992;127(1-4):223-32. doi: 10.1007/BF01309586.
Two monoclonal antibodies (MAbs), 1H10 and 1F10, have been selected against the denatured glycoprotein of the viral haemorrhagic septicaemia virus (VHSV) of salmonids by immunoblotting. Three reference VHSV serotypes (F1, F2, 23.75) and five VHSV isolates from either different host species (trout, salmon, barbel) or geographical locations in Spain reacted with both MAbs by ELISA. In vitro neutralization of all VHSV serotypes but not infectious haematopoietic necrosis virus (IHNV) was obtained only with MAb 1H10. However, when the MAb 1H10 (but not the non-neutralizing Mab 1F10) concentration was below 100 micrograms/ml rather than neutralizing VHSV infectivity, plaque counts increased 2-3-fold. MAb 1H10 is unique in that it has both, an in vitro enhancing infectivity effect (not described yet for VHSV) and it recognizes denatured G protein in contrast to other previously described neutralizing MAbs against VHSV.
通过免疫印迹法筛选出了两种针对鲑鱼病毒性出血性败血症病毒（VHSV）变性糖蛋白的单克隆抗体（MAb），即1H10和1F10。三种参考VHSV血清型（F1、F2、23.75）以及来自西班牙不同宿主物种（鳟鱼、鲑鱼、鲃）或地理位置的五种VHSV分离株通过ELISA与这两种单克隆抗体发生反应。仅用单克隆抗体1H10就实现了对所有VHSV血清型而非传染性造血坏死病毒（IHNV）的体外中和作用。然而，当单克隆抗体1H10（而非非中和性单克隆抗体1F10）的浓度低于100微克/毫升时，噬斑计数非但没有中和VHSV的感染性，反而增加了2至3倍。单克隆抗体1H10的独特之处在于，它既有体外增强感染性的作用（这一作用此前尚未在VHSV中描述过），而且与其他先前描述的针对VHSV的中和性单克隆抗体不同，它能识别变性的G蛋白。
