人类驱动蛋白超家族蛋白成员4与BRCA2相关因子35的关联
Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.
作者信息
Lee Young Mi, Kim Wankee
机构信息
Institute for Medical Sciences, School of Medicine, Ajou University, Suwon 442-749, South Korea.
出版信息
Biochem J. 2003 Sep 1;374(Pt 2):497-503. doi: 10.1042/BJ20030452.
Abstract
A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
摘要
在间期，大部分人类驱动蛋白超家族蛋白成员4（KIF4）与核基质相关联，而一小部分存在于细胞质中。在有丝分裂期间，它在整个过程中都与染色体相关联。在本研究中，我们使用酵母双杂交系统、免疫共沉淀和共分级分离鉴定了一种与KIF4相互作用的蛋白质。该蛋白质是BRCA2相关因子35（BRAF35），含有一个非特异性DNA结合高迁移率族结构域和一个驱动蛋白样卷曲螺旋结构域。这两种蛋白质之间的相互作用似乎是通过它们各自的α-螺旋卷曲螺旋结构域发生的。共分级分离实验表明，KIF4和BRAF35存在于一个约540 kDa的复合物中。该复合物的组成和生物学意义应进一步研究。
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