Kikuchi Kentaro, Tanaka Atsushi, Miyakawa Hiroshi, Kawashima Yumi, Kawaguchi Naomi, Matsushita Masanao, Gershwin M Eric
Fourth Department of Internal Medicine, Teikyo University School of Medicine, 213-8507, Kanagawa, Japan
Hepatol Res. 2003 Jun;26(2):87-90. doi: 10.1016/s1386-6346(03)00012-3.
Early T-lymphocyte activation 1 (Eta-1)/osteopontin is a soluble ligand with pleomorphic immunologic activities including activation of macrophage chemotaxis, promotion of Th1 responses, and activation of B1 B-cells. A recent study suggested that a single-nucleotide polymorphism (SNP) at position nt 9250 (C to T) in exon 7 was highly associated with systemic lupus erythematosus (SLE). Eta-1/osteopontin was reported to be highly expressed in the MRL/lpr mouse, which is recognized as one of the spontaneous autoimmune models of SLE. In the present study, we first investigated the association with this SNP and susceptibility to primary biliary cirrhosis (PBC). The allele frequencies of C/C, C/T, and T/T at position nt 9250 on the Eta-1/osteopontin gene in 50 PBC patients were 20, 32, and 48%, respectively, compared with 9, 47, and 44% in 34 healthy controls (P<0.16-0.72). The gene frequencies of C and T at this position in such PBC patients were 0.36 and 0.64, whereas those in the healthy controls were 0.32 and 0.68 (P<0.91), respectively. Moreover, clinical findings and pathologic stages were not correlated with the variation of SNP. Those findings suggest no associations with Eta-1/osteopontin genetic polymorphism and susceptibility to PBC.
早期T淋巴细胞激活因子1（Eta-1）/骨桥蛋白是一种具有多种免疫活性的可溶性配体，包括激活巨噬细胞趋化性、促进Th1反应以及激活B1 B细胞。最近一项研究表明，第7外显子nt 9250位置（C到T）的单核苷酸多态性（SNP）与系统性红斑狼疮（SLE）高度相关。据报道，Eta-1/骨桥蛋白在MRL/lpr小鼠中高表达，该小鼠被认为是SLE的自发性自身免疫模型之一。在本研究中，我们首先调查了该SNP与原发性胆汁性肝硬化（PBC）易感性之间的关联。50例PBC患者中，Eta-1/骨桥蛋白基因nt 9250位置的C/C、C/T和T/T等位基因频率分别为20%、32%和48%，而34例健康对照中的频率分别为9%、47%和44%（P<0.16 - 0.72）。此类PBC患者中该位置C和T的基因频率分别为0.36和0.64，而健康对照中分别为0.32和0.68（P<0.91）。此外，临床发现和病理分期与SNP的变异无关。这些结果表明Eta-1/骨桥蛋白基因多态性与PBC易感性之间无关联。
