Liu Xiao-Yi, Li Lei, Yao Jia-Qi, Chen Xi, Liu Qing-Huai
Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Int J Ophthalmol. 2011;4(4):406-9. doi: 10.3980/j.issn.2222-3959.2011.04.17. Epub 2011 Aug 18.
To analyze osteopontin (OPN) expression in vitreous and proliferative retinal membranes of patients with proliferative vitreous retinopathy (PVR).
A total of 54 vitreous fluid samples were obtained between 2009 and 2010, which contained 45 with PVR (group A) and 9 without PVR (group B). Enzyme-linked immunosorbent assay was applied to quantify the OPN concentrations in vitreous fluid. Four samples of proliferative retinal membrane were also obtained at the time of vitrectomy, and their contents of OPN were measured by Real-time RT-PCR.
The OPN levels in the vitreous fluid were 778.48±62.06ng/mL in group A and 452.99±32.52ng/mL in group B. The vitreous OPN levels in group A were significantly higher than those in group B and to rise by time in the early stages of PVR. The average OPN levels in the proliferative retinal membranes (F=0.14) were also higher than those in the retinal pigment cells (F=0) using Real-time RT-PCR.
The high vitreous and proliferative retinal membrane OPN levels in PVR suggest that OPN might promote the development of PVR. The vitreous OPN concentrations are rising by the time in the early phases of PVR.
分析增殖性玻璃体视网膜病变（PVR）患者玻璃体及增殖性视网膜膜中骨桥蛋白（OPN）的表达情况。
于2009年至2010年间共获取54份玻璃体样本，其中45份患有PVR（A组），9份未患PVR（B组）。采用酶联免疫吸附测定法对玻璃体中的OPN浓度进行定量分析。在玻璃体切割术时还获取了4份增殖性视网膜膜样本，并通过实时逆转录聚合酶链反应检测其OPN含量。
A组玻璃体中OPN水平为778.48±62.06ng/mL，B组为452.99±32.52ng/mL。A组玻璃体中的OPN水平显著高于B组，且在PVR早期随时间升高。使用实时逆转录聚合酶链反应检测发现，增殖性视网膜膜中的平均OPN水平（F = 0.14）也高于视网膜色素细胞中的水平（F = 0）。
PVR患者玻璃体及增殖性视网膜膜中OPN水平升高提示OPN可能促进PVR的发展。在PVR早期，玻璃体中OPN浓度随时间升高。
