Alexandrescu M, Ene M, Moroşanu V
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1975 Jul-Sep;20(3):171-82.
The relationship between the main serum immunoglobulin classes (IgM, IgG) and the positive diagnostic serologic tests (hemagglutination-inhibition and complement fixation) followed up in 30 paired serum samples from patients in the course of a primary influenza infection with B virus strain, before and after treatment with 2-ME, showed actual participation of the IgM molecules sensitive to 2-ME in the CF test. HI with the serum fractions of patients and convalescents of influenza B, in the presence of virus strains A and B, that had circulated earlier in the area, showed anamnestic responses by the presence of IgG and IgA antibodies in the patients' sera; HI and CF with the same serum fractions in the presence of virus B/Iaşi/74 of the epidemic, showed the presence of IgM, IgG and IgA in the convalescent sera, as also demonstrated by immunoprecipitation in agar gel with specific IgM, and IgA antisera and by treatment with 2-ME. Separation of these sera on a Sephadex G-200 column revealed filtration of IgM antibodies at the same peak as the IgG and IgA antibodies, possessing a closely resembling molecular weight (7S) and probably being IgM subunits with an immunologic behaviour similar to that of the intact molecule sensitive to 2-ME.
在30例感染B病毒株的原发性流感患者病程中，于2 - ME治疗前后采集的配对血清样本中，对主要血清免疫球蛋白类别（IgM、IgG）与阳性诊断血清学检测（血凝抑制和补体结合）之间的关系进行了研究。结果显示，对2 - ME敏感的IgM分子实际参与了补体结合试验。在存在该地区早期流行的A和B病毒株的情况下，用流感B患者及恢复期患者的血清组分进行血凝抑制试验，结果显示患者血清中存在IgG和IgA抗体，呈现回忆反应；在存在流行株B/Iaşi/74的情况下，用相同血清组分进行血凝抑制试验和补体结合试验，结果显示恢复期血清中存在IgM、IgG和IgA，这也通过用特异性IgM和IgA抗血清进行琼脂凝胶免疫沉淀以及用2 - ME处理得到证实。在Sephadex G - 200柱上对这些血清进行分离，结果显示IgM抗体与IgG和IgA抗体在同一峰处被滤过，其分子量（7S）相近，可能是具有与对2 - ME敏感的完整分子相似免疫行为的IgM亚基。
