Ali J, Rahbar S, Burjaq H, Sultan A M, Al Flamerzi M, Shahata M A M
Assisted Conception Unit, Women's Hospital, Hamad Medical Corporation, Doha, Qatar.
J Assist Reprod Genet. 2003 May;20(5):177-81. doi: 10.1023/a:1023665909123.
To elucidate the appropriateness of current indications for assisted hatching (AH) in cleavage stage human embryos and to confirm our preliminary findings that only young patients (about 67%) benefit from AH.
Prior to transfer, 2 of 3 embryos selected for ET were subjected to laser assisted hatching (LAH). Control group consisted of patients matched by similar characteristics and protocol except LAH was not performed.
The clinical pregnancy rate in women < or = 36 years was 64.9% (24/37) for embryos subjected to LAH but was significantly lower (p = 0.029) in the control (33.3%; 10/30). The implantation rate in women < or = 36 years in the test group was 38.1% (40/105) that was significantly higher than that of the control group (17.5%, 14/80; p = 0.0039).
LAH is beneficial for women < or = 36 years but not for women > or = 37 years, for embryos with thin zonae (< or = 16 micron) but not with thick zonae (> or = 17 micron), and for those with repeated failures (37-50%).
阐明目前人类卵裂期胚胎辅助孵化（AH）指征的合理性，并证实我们之前的发现，即只有年轻患者（约67%）能从AH中获益。
在胚胎移植前，选择用于胚胎移植（ET）的3个胚胎中的2个进行激光辅助孵化（LAH）。对照组由特征和方案相似但未进行LAH的患者组成。
年龄≤36岁的女性中，接受LAH的胚胎临床妊娠率为64.9%（24/37），但对照组显著更低（p = 0.029；33.3%，10/30）。试验组年龄≤36岁女性的着床率为38.1%（40/105），显著高于对照组（17.5%，14/80；p = 0.0039）。
LAH对年龄≤36岁的女性有益，但对年龄≥37岁的女性无益；对透明带薄（≤16微米）的胚胎有益，但对透明带厚（≥17微米）的胚胎无益；对有反复失败情况（37 - 50%）的胚胎有益。
