Razi Mohammad Hossein, Halvaei Iman, Razi Yasamin
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2013 Dec;11(12):1021-6.
Routine use of assisted hatching (AH) following ICSI is a controversial issue in the literature. There are rare studies regarding the effect of laser assisted hatching (LAH) on live birth rate.
Our main goal was to evaluate the effect of LAH on delivery rate as well as congenital anomaly in patients undergoing their first ICSI cycle.
A total of 182 patients subjected to ICSI were randomly aliquot into two groups of experiment and control. In experiment group, the embryos were subjected to LAH to open a hole in ZP (about 10-12 µm) while in control group, the transferred embryos were intact with no AH. The patients were followed for clinical pregnancy and delivery rate as well as congenital anomaly. All the patients were infertile due to male factor infertility and LAH and embryo transfer were done on day 2.
Laboratory and clinical characteristics of two groups of experiment and control were the same. There were insignificant differences between two groups of experiment and control for clinical pregnancy rate (20% vs. 23.9%, respectively, p=0.3) and live birth rate (11.11% vs. 8.6%, respectively, p=0.6). Also no significant differences were observed between two groups of experiment and control for multiple pregnancy as well as congenital anomaly.
Routine use of LAH in first ICSI cycle for male factor patients may have no beneficial effects on clinical pregnancy and live birth rate.
在卵胞浆内单精子注射（ICSI）后常规使用辅助孵化（AH）在文献中是一个有争议的问题。关于激光辅助孵化（LAH）对活产率影响的研究很少。
我们的主要目标是评估LAH对接受首次ICSI周期的患者的分娩率以及先天性异常的影响。
总共182例接受ICSI的患者被随机等分为实验组和对照组。在实验组中，对胚胎进行LAH以在透明带（ZP）上打开一个孔（约10 - 12微米），而在对照组中，移植的胚胎完整，未进行AH。对患者进行临床妊娠、分娩率以及先天性异常的随访。所有患者均因男性因素不育，LAH和胚胎移植均在第2天进行。
实验组和对照组的实验室和临床特征相同。实验组和对照组在临床妊娠率（分别为20%和23.9%，p = 0.3）和活产率（分别为11.11%和8.6%，p = 0.6）方面无显著差异。两组在多胎妊娠以及先天性异常方面也未观察到显著差异。
对于男性因素患者，在首次ICSI周期中常规使用LAH可能对临床妊娠和活产率没有有益影响。
