Terpstra Melissa, Henry Pierre-Gilles, Gruetter Rolf
University of Minnesota, Department of Radiology, Center for MR Research Minneapolis, Minnesota.
Magn Reson Med. 2003 Jul;50(1):19-23. doi: 10.1002/mrm.10499.
The concentration of reduced glutathione (GSH), an antioxidant, may be altered in various brain diseases. MEGA-PRESS was used to edit for the (1)H NMR signal from GSH in the occipital lobe of 12 normal humans. In all studies, GSH was clearly detected with a spectral pattern consistent with spectra acquired from a phantom containing GSH. Retention of singlet resonances in the subspectra, a key advantage of this difference-editing technique, provided an unambiguous reference for the offset and phase of the edited signal. Linear combination model (LCModel) analysis provided an unbiased means for quantifying signal contribution from edited metabolites. GSH concentration was estimated from the in vivo spectra as 1.3 +/- 0.2 micro mol/g (mean +/- SD, n = 12).
还原型谷胱甘肽（GSH）作为一种抗氧化剂，其浓度在各种脑部疾病中可能会发生改变。采用MEGA-PRESS对12名正常人枕叶中GSH的氢核磁共振信号进行编辑。在所有研究中，均能清晰检测到GSH，其光谱模式与从含有GSH的模型中获取的光谱一致。子光谱中单线共振的保留是这种差异编辑技术的一个关键优势，为编辑信号的偏移和相位提供了明确的参考。线性组合模型（LCModel）分析为量化编辑代谢物的信号贡献提供了一种无偏方法。根据体内光谱估计GSH浓度为1.3±0.2微摩尔/克（平均值±标准差，n = 12）。
