Aktan Bülent, Taysi Seyithan, Gumustekin Kenan, Bakan Nuri, Sutbeyaz Yavuz
Department of Otorhinolaryngology-Head and Neck Surgery, Atatürk University Medical School, Erzurum, Turkey.
Ann Clin Lab Sci. 2003 Spring;33(2):232-6.
Oxygen free radicals (OFRs) have been implicated in the pathogenesis of an increasing number of diseases and inflammatory states. They may cause cell and tissue damage by their chemical modification of proteins, carbohydrates, nucleotides, and lipids. Under physiological conditions OFRs are part of normal regulatory circuits and are neutralized by antioxidants. Infections are one cause of increased OFR production. The aims of our study were to assess whether the increased oxidative stress in experimental otitis media with effusion (OME) is reflected in erythrocytes by lipid peroxidation and to survey the alterations in oxidant and antioxidant enzyme activities in experimental OME in guinea pigs. Erythrocyte total (enzymatic plus non-enzymatic) superoxide scavenger activity (TSSA), non-enzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD), catalase (CAT), and xanthine oxidase (XO) activities, and malondialdehyde (MDA) level were measured in 6 guinea pigs with OME and in 6 controls. The TSSA, SOD, XO activities, and MDA level in experimental OME were significantly higher than in controls. No significant differences were found in erythrocyte NSSA and CAT activities. In experimental OME induced by histamine injection, increased OFR production was observed, suggesting that OFRs may play an important role in cell and tissue damage due to OME.
氧自由基（OFRs）与越来越多的疾病和炎症状态的发病机制有关。它们可能通过对蛋白质、碳水化合物、核苷酸和脂质进行化学修饰而导致细胞和组织损伤。在生理条件下，氧自由基是正常调节回路的一部分，并被抗氧化剂中和。感染是氧自由基产生增加的一个原因。我们研究的目的是评估实验性中耳积液（OME）中氧化应激增加是否通过脂质过氧化反映在红细胞中，并调查豚鼠实验性OME中氧化剂和抗氧化酶活性的变化。测量了6只患有OME的豚鼠和6只对照豚鼠的红细胞总（酶促加非酶促）超氧化物清除活性（TSSA）、非酶促超氧化物清除活性（NSSA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和黄嘌呤氧化酶（XO）活性以及丙二醛（MDA）水平。实验性OME中的TSSA、SOD、XO活性和MDA水平显著高于对照组。红细胞NSSA和CAT活性未发现显著差异。在通过注射组胺诱导的实验性OME中，观察到氧自由基产生增加，这表明氧自由基可能在OME导致的细胞和组织损伤中起重要作用。
