Maurer Martin H, Feldmann Robert E, Fütterer Carsten D, Kuschinsky Wolfgang
Dept, of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany.
Proteome Sci. 2003 Jun 12;1(1):4. doi: 10.1186/1477-5956-1-4.
Hippocampal neural stem cells (HNSC) play an important role in cerebral plasticity in the adult brain and may contribute to tissue repair in neurological disease. To describe their biological potential with regard to plasticity, proliferation, or differentiation, it is important to know the cellular composition of their proteins, subsumed by the term proteome. RESULTS: Here, we present for the first time a proteomic database for HNSC isolated from the brains of adult rats and cultured for 10 weeks. Cytosolic proteins were extracted and subjected to two-dimensional gel electrophoresis followed by protein identification through mass spectrometry, database search, and gel matching. We could map about 1141 PlusMinus; 209 (N = 5) protein spots for each gel, of which 266 could be identified. We could group the identified proteins into several functional categories including metabolism, protein folding, energy metabolism and cellular respiration, as well as cytoskeleton, Ca2+ signaling pathways, cell cycle regulation, proteasome and protein degradation. We also found proteins belonging to detoxification, neurotransmitter metabolism, intracellular signaling pathways, and regulation of DNA transcription and RNA processing. CONCLUSIONS: The HNSC proteome database is a useful inventory which will allow to specify changes in the cellular protein expression pattern due to specific activated or suppressed pathways during differentiation or proliferation of neural stem cells. Several proteins could be identified in the HNSC proteome which are related to differentiation and plasticity, indicating activated functional pathways. Moreover, we found a protein for which no expression has been described in brain cells before.
海马神经干细胞（HNSC）在成体大脑的脑可塑性中发挥重要作用，可能有助于神经疾病中的组织修复。为了描述它们在可塑性、增殖或分化方面的生物学潜能，了解其蛋白质的细胞组成（即蛋白质组）非常重要。
在此，我们首次展示了一个从成年大鼠大脑中分离并培养10周的HNSC蛋白质组数据库。提取胞质蛋白，进行二维凝胶电泳，然后通过质谱、数据库搜索和凝胶匹配进行蛋白质鉴定。每张凝胶我们可以定位约1141±209（N = 5）个蛋白点，其中266个可以被鉴定。我们可以将鉴定出的蛋白质分为几个功能类别，包括代谢、蛋白质折叠、能量代谢和细胞呼吸，以及细胞骨架、Ca2+信号通路、细胞周期调控、蛋白酶体和蛋白质降解。我们还发现了属于解毒、神经递质代谢、细胞内信号通路以及DNA转录和RNA加工调控的蛋白质。
HNSC蛋白质组数据库是一个有用的资源库，它将有助于明确神经干细胞在分化或增殖过程中由于特定激活或抑制途径导致的细胞蛋白质表达模式的变化。在HNSC蛋白质组中可以鉴定出几种与分化和可塑性相关的蛋白质，表明功能途径被激活。此外，我们发现了一种以前在脑细胞中未描述过表达的蛋白质。
