Moore P S, Pizza C
Medical Research Council, Collaborative Centre, Mill Hill, London, U.K.
Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):717-9. doi: 10.1042/bj2880717.
The sensitivity and specificity of the inhibition of HIV-1 reverse transcriptase by various catechins have been examined. As previously reported, (-)epicatechin 3-gallate inhibits the viral polymerase. However, it is noted here that this inhibition is not observed in the presence of either serum albumin or Triton X-100. Other catechins behave similarly to (-)epicatechin 3-gallate in that they inhibit polymerase activity only in the absence of these reagents. Additionally, other DNA polymerases are inhibited to a similar degree by (-)epicatechin 3-gallate. Taken cumulatively, these results suggest that these catechins, and in particular (-)epicatechin 3-gallate, bind with no apparent selectivity and that the observed inhibition of HIV-1 reverse transcriptase is non-specific in nature.
已对各种儿茶素抑制HIV-1逆转录酶的敏感性和特异性进行了研究。如先前报道，（-）表儿茶素3-没食子酸酯可抑制病毒聚合酶。然而，此处应注意的是，在血清白蛋白或Triton X-100存在的情况下未观察到这种抑制作用。其他儿茶素的行为与（-）表儿茶素3-没食子酸酯相似，即它们仅在不存在这些试剂的情况下抑制聚合酶活性。此外，（-）表儿茶素3-没食子酸酯对其他DNA聚合酶的抑制程度相似。综合来看，这些结果表明这些儿茶素，尤其是（-）表儿茶素3-没食子酸酯，没有明显的选择性结合，并且观察到的对HIV-1逆转录酶的抑制本质上是非特异性的。
