Webersinke G, Bauer H, Amberger A, Zach O, Bauer H C
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):877-84. doi: 10.1016/0006-291x(92)92285-6.
By use of random-primed cDNA probes the expression of extracellular matrix molecules in cerebral microvascular endothelial cells (cEC) and in astrocytes from mouse brain was examined. Two phenotypically different batches of cloned cEC were used. Expression of major adhesive ECM molecules, constituting the endothelial basement membrane (i.e., fibronectin, laminin A, B and collagen IV) and of other attachment factors, such as SPARC (osteonectin), tenascin and thrombospondin 1, was examined. We have demonstrated that cEC of different morphology display variations in the expression of fibronectin (FN), thrombospondin 1 (TSP1) and collagen IV (C IV). Astrocytes were shown to contain FN, TSP1, TN and SPARC mRNA. Unexpectedly, SPARC mRNA could not be detected in any of the capillary endothelial cells examined. Therefore, we suggest that astrocytes are likely to be involved in endothelial differentiation and function in the central nervous system via ECM molecule secretion.
利用随机引物cDNA探针，检测了小鼠脑微血管内皮细胞（cEC）和星形胶质细胞中细胞外基质分子的表达。使用了两批表型不同的克隆cEC。检测了构成内皮基底膜的主要黏附性细胞外基质分子（即纤连蛋白、层粘连蛋白A、B和IV型胶原）以及其他黏附因子，如富含半胱氨酸的酸性分泌蛋白（骨连接蛋白）、腱生蛋白和血小板反应蛋白1的表达。我们已经证明，不同形态的cEC在纤连蛋白（FN）、血小板反应蛋白1（TSP1）和IV型胶原（C IV）的表达上存在差异。星形胶质细胞被证明含有FN、TSP1、TN和SPARC mRNA。出乎意料的是，在所检测的任何毛细血管内皮细胞中均未检测到SPARC mRNA。因此，我们认为星形胶质细胞可能通过分泌细胞外基质分子参与中枢神经系统内皮细胞的分化和功能。
