Tan E M, Glassberg E, Olsen D R, Noveral J P, Unger G A, Peltonen J, Chu M L, Levine E, Sollberg S
Department of Pathology, Thomas Jefferson University, Philadelphia, PA.
Matrix. 1991 Dec;11(6):380-7. doi: 10.1016/s0934-8832(11)80192-7.
In this study, the expression of extracellular matrix genes by vascular cells from human iliac blood vessels was characterized on the mRNA steady-state level by slot blot and Northern transfer analyses, as well as by in situ hybridization. Endothelial cells were isolated from adult human iliac arteries and veins, as well as from umbilical veins; smooth muscle cells were isolated from adult human iliac arteries and inferior vena cava. The results show that confluent umbilical vein endothelial cells expressed the genes that encode types I, III, IV and VI collagens, as well as fibronectin and laminin. In contrast, the iliac endothelial cells expressed the genes for types IV and V collagens, fibronectin and laminin; mRNA transcripts for types I, III and VI collagens were not detectable. The smooth muscle cells from iliac arteries or inferior vena cava displayed gene expression for types I, III, IV, V and VI collagens, fibronectin and laminin. The results indicate major differences in gene expression for the various types of collagens by human iliac endothelial and smooth muscle cells. Furthermore, the fetal-derived umbilical endothelial cells displayed differential collagen gene expression from that of adult iliac endothelial cells.
在本研究中，通过狭缝印迹和Northern印迹分析以及原位杂交，在mRNA稳态水平上对来自人髂血管的血管细胞的细胞外基质基因表达进行了表征。内皮细胞从成人髂动脉和静脉以及脐静脉中分离得到；平滑肌细胞从成人髂动脉和下腔静脉中分离得到。结果表明，汇合的脐静脉内皮细胞表达编码I型、III型、IV型和VI型胶原蛋白以及纤连蛋白和层粘连蛋白的基因。相比之下，髂内皮细胞表达IV型和V型胶原蛋白、纤连蛋白和层粘连蛋白的基因；未检测到I型、III型和VI型胶原蛋白的mRNA转录本。来自髂动脉或下腔静脉的平滑肌细胞显示出I型、III型、IV型、V型和VI型胶原蛋白、纤连蛋白和层粘连蛋白的基因表达。结果表明，人髂内皮细胞和平滑肌细胞在各种类型胶原蛋白的基因表达上存在主要差异。此外，胎儿来源的脐内皮细胞与成人髂内皮细胞的胶原蛋白基因表达不同。
