Jacobsen C S, Pedersen J C
National Environmental Research Institute, Department of Marine Ecology and Microbiology, Roskilde, Denmark.
Biodegradation. 1991;2(4):245-52. doi: 10.1007/BF00114556.
The 2,4-dichlorophenoxyacetic acid (2,4-D) degrading pseudomonad, Pseudomonas cepacia DBO1(pRO101), was inoculated at approximately 10(7) CFU/g into sterile and non-sterile soil amended with 0, 5 or 500 ppm 2,4-D and the survival of the strain was studied for a period of 44 days. In general, the strain survived best in sterile soil. When the sterile soil was amended with 2,4-D, the strain survived at a significantly higher level than in non-amended sterile soil. In non-sterile soil either non-amended or amended with 5 ppm 2,4-D the strain died out, whereas with 500 ppm 2,4-D the strain only declined one order of magnitude through the 44 days. The influence of 0, 0.06, 12 and 600 ppm 2,4-D on short-term (48 h) survival of P. cepacia DBO1(pRO101) inoculated to a level of 6 x 10(4), 6 x 10(6) or 1 x 10(8) CFU/g soil was studied in non-sterile soil. Both inoculum level and 2,4-D concentration were found to have a positive influence on numbers of P. cepacia DBO1(pRO101). At 600 ppm 2,4-D growth was significant irrespective of the inoculation level, and at 12 ppm growth was stimulated at the two lowest inocula levels. P. cepacia DBO1(pRO101) was able to survive for 15 months in sterile buffers kept at room temperature. During this starvation, cells shrunk to about one third the volume of exponentially growing cells.
将2,4-二氯苯氧乙酸（2,4-D）降解假单胞菌洋葱伯克霍尔德菌DBO1（pRO101）以约10⁷CFU/g的浓度接种到添加了0、5或500 ppm 2,4-D的无菌和非无菌土壤中，并对该菌株的存活情况进行了44天的研究。总体而言，该菌株在无菌土壤中存活得最好。当向无菌土壤中添加2,4-D时，该菌株的存活水平明显高于未添加2,4-D的无菌土壤。在未添加或添加了5 ppm 2,4-D的非无菌土壤中，该菌株死亡，而在添加了500 ppm 2,4-D的非无菌土壤中，该菌株在44天内仅下降了一个数量级。研究了0、0.06、12和600 ppm 2,4-D对接种量为6×10⁴、6×10⁶或1×10⁸CFU/g土壤的洋葱伯克霍尔德菌DBO1（pRO101）在非无菌土壤中的短期（48小时）存活情况的影响。发现接种量和2,4-D浓度对洋葱伯克霍尔德菌DBO1（pRO101）的数量均有积极影响。在浓度为600 ppm 2,4-D时，无论接种量如何，生长都很显著；在浓度为12 ppm时，在两个最低接种量水平下生长受到刺激。洋葱伯克霍尔德菌DBO1（pRO101）能够在室温下的无菌缓冲液中存活15个月。在这种饥饿状态下，细胞收缩至指数生长期细胞体积约三分之一大小。
