Havel J, Reineke W
Bergische Universität, Gesamthochschule Wuppertal, Chemische Mikrobiologie, FRG.
FEMS Microbiol Lett. 1993 Apr 1;108(2):211-7. doi: 10.1111/j.1574-6968.1993.tb06101.x.
The hybrid Pseudomonas cepacia strain JHR22 was tested for its ability to degrade Aroclor 1221 in soil. The influence of supplements--mineral salts and trace elements--on the degradation was investigated. Disappearance of Aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under different conditions. After 45 days the hybrid organism, strain JHR22, was still present at high numbers in soil, independently of whether the soil had been sterilized prior to inoculation or not. There was only a minor difference in degradation efficiency between sterilized and untreated soil with about 70% release of chloride when 10(7) cells/g soil were inoculated. The whole hybrid pathway, originating from three different strains, was found to be stable under the conditions tested. Mineral salts did not significantly affect the degradation rate or survival of the hybrid strain.
对杂交洋葱伯克霍尔德菌菌株JHR22在土壤中降解多氯联苯混合物Aroclor 1221的能力进行了测试。研究了矿物质盐和微量元素等添加物对降解的影响。在不同条件下测定了Aroclor 1221同系物的消失情况、代谢产物的产生以及氯的释放。45天后，无论接种前土壤是否经过灭菌处理，杂交菌株JHR22在土壤中仍大量存在。当每克土壤接种10(7)个细胞时，灭菌土壤和未处理土壤之间的降解效率只有微小差异，氯的释放量约为70%。发现源自三种不同菌株的整个杂交途径在测试条件下是稳定的。矿物质盐对杂交菌株的降解速率或存活没有显著影响。
