太平洋牡蛎（Crassostrea gigas）中热休克同源蛋白70（hsc70）和热休克蛋白70（hsp70）基因的分子鉴定与表达
Molecular identification and expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70) genes in the Pacific oyster Crassostrea gigas.
作者信息
Boutet Isabelle, Tanguy Arnaud, Rousseau Sabrina, Auffret Michel, Moraga Dario
机构信息
Laboratoire de Sciences de l'Environnement Marin, UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Nicolas Copernic, 29280, Plouzané, France.
出版信息
Cell Stress Chaperones. 2003 Spring;8(1):76-85. doi: 10.1379/1466-1268(2003)8<76:miaeoh>2.0.co;2.
Abstract
The 70-kDa heat shock protein (Hsp) family is composed of both environmentally inducible (Hsp) and constitutively expressed (Hsc) family members. We sequenced 2 genes encoding an Hsp70 and an Hsc70 in the Pacific oyster Crassostrea gigas. The Cghsc70 gene contained introns, whereas the Cghsp70 gene did not. Moreover, the corresponding amino acid sequences of the 2 genes presented all the characteristic motifs of the Hsp70 family. We also investigated the expression of Hsp70 in tissues of oysters experimentally exposed to metal. A recombinant Hsc72 was used as an antigen to produce a polyclonal antibody to quantify soluble Hsp70 by enzyme-linked immunosorbent assay in protein samples extracted from oysters. Our results showed that metals (copper and cadmium) induced a decrease in cytosolic Hsp70 level in gills and digestive gland of oysters experimentally exposed to metal. These data suggest that metals may inhibit stress protein synthesis.
摘要
70千道尔顿热休克蛋白（Hsp）家族由环境诱导型（Hsp）和组成型表达型（Hsc）家族成员组成。我们对太平洋牡蛎（Crassostrea gigas）中编码一种Hsp70和一种Hsc70的两个基因进行了测序。Cghsc70基因含有内含子，而Cghsp70基因没有。此外，这两个基因的相应氨基酸序列呈现出Hsp70家族的所有特征基序。我们还研究了实验性暴露于金属的牡蛎组织中Hsp70的表达。使用重组Hsc72作为抗原制备多克隆抗体，通过酶联免疫吸附测定法对从牡蛎中提取的蛋白质样品中的可溶性Hsp70进行定量。我们的结果表明，金属（铜和镉）会导致实验性暴露于金属的牡蛎鳃和消化腺中胞质Hsp70水平降低。这些数据表明，金属可能会抑制应激蛋白的合成。
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本文引用的文献
1
Production of antibodies using proteins in gel bands.利用凝胶条带中的蛋白质生产抗体。
Methods Mol Biol. 1988;3:355-62. doi: 10.1385/0-89603-126-8:355.
2
The impact of heavy metals on the grey garden slug, Deroceras reticulatum (Müller): Metal storage, cellular effects and semi-quantitative evaluation of metal toxicity.
Environ Pollut. 1996;93(3):327-43. doi: 10.1016/s0269-7491(96)00048-6.
3
Immunochemical quantification of metallothioneins in marine mollusks: characterization of a metal exposure bioindicator.海洋软体动物中金属硫蛋白的免疫化学定量：一种金属暴露生物指标的特性
Environ Toxicol Chem. 2002 May;21(5):1009-14.
4
Adjusting the thermostat: the threshold induction temperature for the heat-shock response in intertidal mussels (genus Mytilus) changes as a function of thermal history.调节恒温器：潮间带贻贝（紫贻贝属）热休克反应的阈值诱导温度随热历史而变化。
J Exp Biol. 2001 Oct;204(Pt 20):3571-9. doi: 10.1242/jeb.204.20.3571.
5
Evaluation for Hsp70 as a biomarker of effect of pollutants on the earthworm Lumbricus terrestris.评估热休克蛋白70作为污染物对蚯蚓（陆正蚓）影响的生物标志物。
Cell Stress Chaperones. 2001 Apr;6(2):153-63. doi: 10.1379/1466-1268(2001)006<0153:efhaab>2.0.co;2.
6
Induction of marine mollusc stress proteins by chemical or physical stress.
Arch Environ Contam Toxicol. 2001 Jul;41(1):22-9. doi: 10.1007/s002440010217.
7
Characterization of a cDNA encoding a 72 kDa heat shock cognate protein (Hsc72) from the Pacific oyster, Crassostrea gigas.太平洋牡蛎（Crassostrea gigas）中编码72 kDa热休克同源蛋白（Hsc72）的cDNA的鉴定。
DNA Seq. 2000;11(3-4):265-70. doi: 10.3109/10425170009033241.
8
Cloning and sequence analysis of a cDNA for an inducible 70 kDa heat shock protein (Hsp70) of the American oyster (Crassostrea virginica).
DNA Seq. 2000;11(3-4):261-4. doi: 10.3109/10425170009033240.
9
Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors.
Aquat Toxicol. 2001 Jan;51(3):277-91. doi: 10.1016/s0166-445x(00)00119-3.
10
Cloning and analysis of a Trichinella britovi gene encoding a cytoplasmic heat shock protein of 72 kDa.
Parasitology. 1999 Jul;119 ( Pt 1):81-93. doi: 10.1017/s0031182099004461.
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