亚硫酸氢钠对人淋巴细胞诱导的细胞遗传学损伤。
Cytogenetic damage induced in human lymphocytes by sodium bisulfite.
作者信息
Meng Z, Zhang L
机构信息
Department of Environmental Science, Shanxi University, Taiyuan, China.
出版信息
Mutat Res. 1992 Dec;298(2):63-9. doi: 10.1016/0165-1218(92)90030-4.
The frequencies of chromosomal aberrations (CA), sister-chromatid exchanges (SCE), and micronuclei (MN) in human blood lymphocytes exposed to sodium bisulfite (sulfur dioxide) at various concentrations ranging from 5 x 10(-5) M to 2 x 10(-3) M in vitro were studied. It was shown that sodium bisulfite (NaHSO3 and Na2SO3, 1:3 M/M) caused an increase in SCE and MN in human blood lymphocytes in a dose-dependent manner, and also induced mitotic delays and decreased mitotic index. For CA, our results indicated that sodium bisulfite induced an increase of chromatid-type aberrations in lymphocytes from three of four donors in a dose-dependent manner. The chemical at low concentrations induced chromatid-type aberrations, but not chromosome-type aberrations; high concentrations induced both chromatid- and chromosome-type aberrations. No cytogenetic damage in human lymphocytes was induced by sodium sulfate. The results have confirmed that sulfur dioxide is a clastogenic and genotoxic agent.
研究了体外暴露于浓度范围为5×10⁻⁵ M至2×10⁻³ M的亚硫酸氢钠（二氧化硫）的人血淋巴细胞中染色体畸变（CA）、姐妹染色单体交换（SCE）和微核（MN）的频率。结果表明，亚硫酸氢钠（NaHSO₃和Na₂SO₃，1:3 M/M）以剂量依赖性方式导致人血淋巴细胞中SCE和MN增加，还诱导有丝分裂延迟并降低有丝分裂指数。对于CA，我们的结果表明，亚硫酸氢钠以剂量依赖性方式诱导四个供体中三个供体的淋巴细胞中染色单体型畸变增加。低浓度的该化学物质诱导染色单体型畸变，但不诱导染色体型畸变；高浓度则诱导染色单体型和染色体型畸变。硫酸钠未诱导人淋巴细胞的细胞遗传学损伤。结果证实二氧化硫是一种致断裂剂和遗传毒性剂。
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