硼酸对培养的人淋巴细胞姐妹染色单体交换和染色体畸变的影响。
The effects of boric acid on sister chromatid exchanges and chromosome aberrations in cultured human lymphocytes.
机构信息
Department Biology, Natural and Applied Science Institute, Cukurova University, 01330, Adana, Turkey.
出版信息
Cytotechnology. 2008 Feb;56(2):91-6. doi: 10.1007/s10616-007-9094-z. Epub 2007 Dec 28.
The aim of this study was to determine the possible genotoxic effects of boric acid (BA) (E284), which is used as an antimicrobial agent in food, by using sister chromatid exchange (SCEs) and chromosome aberration (CAs) tests in human peripheral lymphocytes. The human lymphocytes were treated with 400, 600, 800, and 1000 mug/mL concentrations of BA dissolved in dimethyl sulfoxide (DMSO), for 24 h and 48 h treatment periods. BA did not increase the SCEs for all the concentrations and treatment periods when compared to control and solvent control (DMSO). BA induced structural and total CAs at all the tested concentrations for 24 and 48 h treatment periods. The induction of the total CAs was dose dependent for the 24 h treatment period. However, BA did not cause numerical CAs. BA showed a cytotoxic effect by decreasing the replication index (RI) and mitotic index (MI). BA decreased the MI in a dose-dependent manner for the 24 h treatment period.
本研究旨在通过人外周血淋巴细胞姐妹染色单体交换（SCE）和染色体畸变（CA）试验，确定硼酸（BA）（E284）作为食品抗菌剂的可能遗传毒性作用。用二甲基亚砜（DMSO）溶解的 400、600、800 和 1000μg/ml 浓度的 BA 处理人淋巴细胞 24 和 48 小时。与对照和溶剂对照（DMSO）相比，BA 并未增加所有浓度和处理时间的 SCE。BA 在所有测试浓度下均诱导结构和总 CA，在 24 和 48 小时处理期。24 小时处理期总 CA 的诱导与剂量呈依赖性。然而，BA 并未导致数值 CA。BA 通过降低复制指数（RI）和有丝分裂指数（MI）表现出细胞毒性作用。BA 降低了 24 小时处理期的 MI，呈剂量依赖性。
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