十二周红细胞储存。
Twelve-week RBC storage.
作者信息
Hess John R, Hill Heather R, Oliver Cindy K, Lippert Lloyd E, Rugg Neeta, Joines Amy D, Gormas Jennifer F, Pratt P Gayle, Silverstein Edward B, Greenwalt Tibor J
机构信息
Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC, USA.
出版信息
Transfusion. 2003 Jul;43(7):867-72. doi: 10.1046/j.1537-2995.2003.00442.x.
BACKGROUND
Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage.
STUDY DESIGN AND METHODS
In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 mL of one of four variants of experimental additive solution 76 (EAS-76) containing 45, 40, 35, or 30 mEq per L NaCl. Units were sampled weekly for 12 weeks for morphologic and biochemical measures. In the second study, 10 volunteers donated 2 units of RBCs for a crossover comparison of Tc/Cr 24-hour in vivo recovery of 6-week storage in AS-1 versus 12-week storage in EAS-76 variant 6 (EAS-76v6) having 30 mEq per L NaCl.
RESULTS
RBCs stored in the lower salt variants of EAS-76 had higher concentrations of RBC ATP with less hemolysis and microvesiculation. RBC 2,3 DPG was preserved for two weeks. RBCs stored for 12 weeks in EAS-76v6 exhibited 78 +/- 4 percent 24-hour in vivo recovery.
CONCLUSIONS
It is possible to store RBCs for 12 weeks with acceptable recovery and 0.6 percent hemolysis and with normal 2,3 DPG concentrations for 2 weeks.
背景
更好的储存方式可提高红细胞的可用性和安全性。优化红细胞的三磷酸腺苷（ATP）生成并将溶血降至最低，已使红细胞的储存时间得以逐步延长。
研究设计与方法
在第一项研究中，将24单位的CPD浓缩红细胞按每组4单位进行汇集、重新分装，并添加到300毫升四种实验添加剂溶液76（EAS - 76）变体之一中，这些变体每升氯化钠（NaCl）含量分别为45、40、35或30毫当量。每周对样本进行一次采样，持续12周，以进行形态学和生化指标检测。在第二项研究中，10名志愿者捐献了2单位红细胞，用于对AS - 1中储存6周与每升含30毫当量NaCl的EAS - 76变体6（EAS - 76v6）中储存12周的红细胞进行锝/铬（Tc/Cr）24小时体内回收率的交叉比较。
结果
储存在EAS - 76低盐变体中的红细胞具有更高的ATP浓度，溶血和微囊泡形成较少。红细胞2,3 - 二磷酸甘油酸（2,3 - DPG）可保持两周。在EAS - 76v6中储存12周的红细胞24小时体内回收率为78±4%。
结论
红细胞有可能储存12周，回收率可接受，溶血率为0.6%，且2,3 - DPG浓度在两周内保持正常。
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