Tidcombe Hester, Jackson-Fisher Amy, Mathers Kathleen, Stern David F, Gassmann Martin, Golding Jon P
Division of Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8281-6. doi: 10.1073/pnas.1436402100. Epub 2003 Jun 24.
Mice lacking the epidermal growth factor receptor family member ErbB4 exhibit defects in cranial neural crest cell migration but die by embryonic day 11 because of defective heart development. To examine later phenotypes, we rescued the heart defects in ErbB4 mutant mice by expressing ErbB4 under a cardiac-specific myosin promoter. Rescued ErbB4 mutant mice reach adulthood and are fertile. However, during pregnancy, mammary lobuloalveoli fail to differentiate correctly and lactation is defective. Rescued mice also display aberrant cranial nerve architecture and increased numbers of large interneurons within the cerebellum.
缺乏表皮生长因子受体家族成员ErbB4的小鼠在颅神经嵴细胞迁移方面表现出缺陷，但由于心脏发育缺陷在胚胎第11天死亡。为了研究后期表型，我们通过在心脏特异性肌球蛋白启动子下表达ErbB4来挽救ErbB4突变小鼠的心脏缺陷。获救的ErbB4突变小鼠能够活到成年且可育。然而，在怀孕期，乳腺小叶腺泡不能正常分化，泌乳功能也存在缺陷。获救小鼠还表现出异常的颅神经结构，并且小脑内大中间神经元数量增加。
