Kovác J, Zachar V, Brůcková M, Ujhélyi E, Füst G, Mayer V, Zajac V
Institute of Virology, Bratislava, Czecho-Slovakia.
Acta Virol. 1992 Aug;36(4):337-46.
Recombinant proteins derived from immunodominant conserved domains of HIV-1 env and gag genes were synthesized in E. coli. An immunoblot system using total cell lysates was employed for the analysis of recombinant bacterial clones. Together 427 serum samples obtained from asymptomatic anti-HIV seropositive individuals, AIDS patients, healthy donors and persons suffering from various conditions were comparatively evaluated for the presence of HIV-1 antibodies using recombinant peptides and commercially available western blot (WB) and ELISA assays. The recombinant antigen product of plasmid pEX41 was found to be superior, with respect to sensitivity and specificity, to the viral gp41 which represents a diagnostically important constituent of the WB.
从HIV-1包膜蛋白和 gag基因的免疫显性保守结构域衍生而来的重组蛋白在大肠杆菌中合成。采用使用全细胞裂解物的免疫印迹系统分析重组细菌克隆。使用重组肽以及市售的蛋白质印迹（WB）和酶联免疫吸附测定（ELISA）试验，对从无症状抗HIV血清阳性个体、艾滋病患者、健康供体以及患有各种病症的人群中获得的427份血清样本进行比较评估，以检测HIV-1抗体的存在。发现质粒pEX41的重组抗原产物在敏感性和特异性方面优于病毒gp41，后者是WB诊断上的重要组成部分。
