“标准酶联免疫吸附测定法”与HIV（env）酶联免疫吸附测定法联合用于检测和确认人血清中的HIV抗体。
The association of "standard ELISA" and HIV (env) ELISA for detection and confirmation of HIV antibodies in human sera.
作者信息
Filice G, Soldini L, Orsolini P, Campisi D, Chiesa A, Gulminetti R, Achilli G, Cattaneo E
机构信息
IRCCS Policlinico S. Matteo, Department of Infectious Diseases, University of Pavia.
出版信息
Microbiologica. 1991 Jan;14(1):9-13.
Sera from various population of subjects, including patients with AIDS and ARC, drug-addicts seropositive for HIV and healthy blood donors were screened with "standard ELISA" and HIV (env) ELISA. In the present study all 80 specimens from patients with AIDS and all 60 patients with ARC were positive by HIV (env) ELISA, and 1507 specimens from HIV drug positive by W.B. were detected as positive by HIV (env) ELISA. The specificity of HIV (env) ELISA was defined in terms of percentage of non-reacting persons in a low risk population is 100%. Furthermore the HIV (env) ELISA is highly specific and sensitive and could be used in association with "standard ELISA" for detection and confirmation of HIV antibodies in human sera and plasma.
使用“标准酶联免疫吸附测定法（ELISA）”和HIV（env）ELISA对来自不同人群的血清进行了筛查，这些人群包括艾滋病患者、艾滋病相关综合征（ARC）患者、HIV血清学阳性的吸毒者以及健康献血者。在本研究中，所有80份艾滋病患者的标本和所有60份ARC患者的标本通过HIV（env）ELISA检测均呈阳性，1507份经免疫印迹法（W.B.）检测为HIV阳性的吸毒者标本通过HIV（env）ELISA检测也呈阳性。HIV（env）ELISA的特异性通过低风险人群中无反应者的百分比来定义，为100%。此外，HIV（env）ELISA具有高度的特异性和敏感性，可与“标准ELISA”联合用于检测和确认人血清和血浆中的HIV抗体。
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