Praveen S Solomon, Hanumantha Ravikumar, Belovich Joanne M, Davis Brian L
Department of Biomedical Engineering, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Diabetes Technol Ther. 2003;5(3):393-9. doi: 10.1089/152091503765691893.
Biocompatibility issues such as protein deposition and fibrous capsule formation significantly reduce the sensitivity of implanted glucose sensors. One of the approaches to improve the sensor biocompatibility is to disguise the sensors with coatings that mimic body conditions. We anticipate that a biomimetic coating based on hyaluronic acid (HA) would minimize the problems related to protein deposition and fibrous tissue formation. Diffusion experiments were conducted to assess the transport properties of HA coating on a polyvinyl alcohol (PVA) membrane using a classic diffusion cell. HA was coated on PVA membranes, as cross-linked HA membranes alone have poor mechanical strength. The effective diffusivities of glucose and oxygen in a HA/PVA membrane (95% confidence interval) are 1 +/- 0.26 x 10(-4) and 1.42 +/- 0.34 x 10(-4) cm(2)/min, respectively. The effective diffusivities of glucose and oxygen in HA/PVA membranes were approximately two-thirds when compared with the diffusivities of glucose and oxygen (7.29 x 10(-5) and 2.34 x 10(-4) cm(2)/min, respectively) in pure PVA membranes. The results indicate that the HA/PVA membranes have transport properties similar to the commonly used pure PVA membranes, and thus may find usefulness as a coating for implantable glucose sensors.
诸如蛋白质沉积和纤维囊形成等生物相容性问题会显著降低植入式葡萄糖传感器的灵敏度。提高传感器生物相容性的方法之一是用模拟人体环境的涂层来伪装传感器。我们预计基于透明质酸（HA）的仿生涂层将最大限度地减少与蛋白质沉积和纤维组织形成相关的问题。使用经典扩散池进行扩散实验，以评估HA涂层在聚乙烯醇（PVA）膜上的传输特性。由于单独的交联HA膜机械强度较差，因此将HA涂覆在PVA膜上。葡萄糖和氧气在HA/PVA膜中的有效扩散系数（95%置信区间）分别为1±0.26×10⁻⁴和1.42±0.34×10⁻⁴ cm²/min。与葡萄糖和氧气在纯PVA膜中的扩散系数（分别为7.29×10⁻⁵和2.34×10⁻⁴ cm²/min）相比，葡萄糖和氧气在HA/PVA膜中的有效扩散系数约为其三分之二。结果表明，HA/PVA膜具有与常用的纯PVA膜相似的传输特性，因此可用作植入式葡萄糖传感器的涂层。
