Zhou Feng C, Sari Youssef, Li Ting-Kai, Goodlett Charles, Azmitia Efrain C
Department of Anatomy and Cell Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA.
Neurotox Res. 2002 Jun;4(4):337-42. doi: 10.1080/10298420290030532.
Serotonin (5-HT) has specific roles not only as a transmitter but also as a signal for differentiation. We recently found that alcohol drinking during pregnancy resulted in incomplete-neural-tube-fusion which hindered the development of midline cells such as 5-HT neurons in mice. We now report that, at the birth of 5-HT neurons (E11), the 5-HT immunoreative (5-HT-im) neurons are often found missing medial projecting fibers towards ventricle in the Alcohol treated group (ALC) as compared with pair-fed (PF) and Chow-fed groups (Chow) in mice. At E13, there are fewer 5-HT-im neurons in either dorsal or median raphe of ALC as compared with PF or Chow; furthermore, neurite outgrowth and migration of the 5-HT neurons are also compromised with alcohol exposure. We, thus, demonstrated that fetal alcohol exposure compromised 5-HT development as early as at the 5-HT neuron birth. Since 5-HT is a signal for development of many forebrain neurons, the deviation of 5-HT in early life may have consequences on brain development that extend beyond those seen in the 5-HT system.
血清素（5-羟色胺，5-HT）不仅作为一种神经递质发挥特定作用，还作为一种分化信号。我们最近发现，孕期饮酒会导致神经管融合不完全，从而阻碍小鼠中诸如5-HT神经元等中线细胞的发育。我们现在报告，在5-HT神经元诞生时（胚胎第11天，E11），与成对喂养（PF）和正常饮食（Chow）组的小鼠相比，在酒精处理组（ALC）中，经常发现5-HT免疫反应性（5-HT-im）神经元向脑室的内侧投射纤维缺失。在胚胎第13天（E13），与PF组或Chow组相比，ALC组背侧或中缝核中的5-HT-im神经元数量更少；此外，5-HT神经元的神经突生长和迁移也因酒精暴露而受到损害。因此，我们证明，胎儿酒精暴露早在5-HT神经元诞生时就损害了5-HT的发育。由于5-HT是许多前脑神经元发育的信号，早期生命中5-HT的偏差可能对大脑发育产生影响，其影响范围可能超出5-HT系统所见的范围。
