Ye W, Shimamura K, Rubenstein J L, Hynes M A, Rosenthal A
Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Cell. 1998 May 29;93(5):755-66. doi: 10.1016/s0092-8674(00)81437-3.
During development, distinct classes of neurons are specified in precise locations along the dorso-ventral and anterior-posterior axes of the neural tube. We provide evidence that intersections of Shh, which is expressed along the ventral neural tube, and FGF8, which is locally produced at the mid/hindbrain boundary and in the rostral forebrain, create induction sites for dopaminergic neurons in the midbrain and forebrain. The same intersection, when preceded by a third signal, FGF4, which is expressed in the primitive streak, defines an inductive center for hindbrain 5-HT neurons. These findings illustrate that cell patterning in the neural plate is a multistep process in which early inducers, which initially divide the neural plate into crude compartments, are replaced by multiple local organizing centers, which specify distinct neuronal cell types within these compartments.
在发育过程中，不同类别的神经元沿着神经管的背腹轴和前后轴在精确的位置被确定。我们提供的证据表明，沿腹侧神经管表达的Shh与在中脑/后脑边界和前脑嘴侧局部产生的FGF8的交汇点，为中脑和前脑的多巴胺能神经元创造了诱导位点。当这个交汇点之前有第三个信号FGF4（在原条中表达）时，它定义了后脑5-羟色胺能神经元的诱导中心。这些发现表明，神经板中的细胞模式形成是一个多步骤过程，其中早期诱导因子最初将神经板分成粗略的区域，随后被多个局部组织中心所取代，这些中心在这些区域内指定不同的神经元细胞类型。
