Kohlmeyer Ute, Jantzen Eckard, Kuballa Jürgen, Jakubik Sandra
GALAB Laboratories, Max-Planck-Strasse 1, 21502 Geesthacht, Germany.
Anal Bioanal Chem. 2003 Sep;377(1):6-13. doi: 10.1007/s00216-003-2064-1. Epub 2003 Jun 27.
A recently developed and validated method for simultaneous determination of 17 inorganic and organic arsenic compounds in marine biota has been successfully applied to routine analysis of different food products, including fish, shellfish, edible algae, rice, and other types of grain. During one year, approximately 250 food samples were analyzed, mostly fish and rice. Long-term stability and robustness of the system was observed and reproducible results for certified reference materials were ensured by means of control charts. The separation was performed by ion-pair chromatography on an anion-exchange column to separate anionic, neutral, and cationic arsenic species in one chromatographic run. Hyphenation to ICP-MS allowed element-specific and sensitive detection of the different arsenic species with a detection limit as low as 8 ng As L(-1 )in the sample extract, which is equivalent to 2 ng As g(-1) in the original sample. Special emphasis was laid on the analysis of marine algae and rice samples. These food types can contain elevated levels of the very toxic inorganic arsenic species (up to 90% in rice) and therefore are the focus of interest in the food industry. In marine algae, inorganic arsenic was mainly present as arsenate whereas in rice arsenite predominated.
一种最近开发并经过验证的用于同时测定海洋生物群中17种无机和有机砷化合物的方法已成功应用于不同食品的常规分析，包括鱼类、贝类、可食用藻类、大米和其他谷物。在一年时间里，大约分析了250个食品样本，主要是鱼类和大米。通过控制图观察到该系统的长期稳定性和稳健性，并确保了认证参考物质的可重现结果。分离是在阴离子交换柱上通过离子对色谱法进行的，以便在一次色谱运行中分离阴离子、中性和阳离子砷物种。与电感耦合等离子体质谱联用允许对不同砷物种进行元素特异性和灵敏检测，在样品提取物中的检测限低至8 ng As L(-1)，相当于原始样品中2 ng As g(-1)。特别强调了对海藻和大米样本的分析。这些食品类型可能含有剧毒无机砷物种的含量升高（大米中高达90%），因此是食品行业关注的焦点。在海藻中，无机砷主要以砷酸盐形式存在，而在大米中砷亚酸盐占主导地位。
