Rey Maria, Pohl Christopher
Dionex Corporation, 1228 Titan Way, Sunnyvale, CA 94085, USA.
J Chromatogr A. 2003 May 16;997(1-2):199-206. doi: 10.1016/s0021-9673(03)00113-4.
The term amines encompasses a wide variety of compounds: monovalent or polyvalent amines, hydrophobic or hydrophilic amines, and combinations of all of these. Due to their charge, polyvalent amines such as the biogenic amines have very strong cation-exchange interaction with the cation-exchange groups in the stationary phase. Very high acid concentrations are required to elute them effectively from a high-capacity, carboxylated cation-exchange column. The eluent must contain a divalent ion to elute them from a sulfonated cation-exchange column due to its selectivity. Chromatography for these amines with a new "tailored" amine column of moderate capacity using a simple acidic eluent is described. The hydrophobic nature of other amines, such as long-carbon-chained amines, results in partitioning into the polymeric substrate of previous carboxylated stationary phases, so that organic solvent is required to elute them effectively. The substrate resin of this new "tailored" amine column is first coated so that, when it is functionalized in a subsequent step, this type of interaction is minimized. Examples are given. Methods that require eluent gradients and/or step changes of eluent concentration are especially well suited to this column because the background conductivity remains almost unchanged under gradient conditions.
单价或多价胺、疏水性或亲水性胺，以及所有这些的组合。由于其电荷，多价胺如生物胺与固定相中的阳离子交换基团具有非常强的阳离子交换相互作用。需要非常高的酸浓度才能从高容量的羧化阳离子交换柱中有效地洗脱它们。由于其选择性，洗脱液必须含有二价离子才能从磺化阳离子交换柱中洗脱它们。本文描述了使用简单酸性洗脱液，通过具有中等容量的新型“定制”胺柱对这些胺进行色谱分析的方法。其他胺类，如长碳链胺的疏水性导致它们分配到先前羧化固定相的聚合物基质中，因此需要有机溶剂才能有效地洗脱它们。这种新型“定制”胺柱的基质树脂首先进行涂覆，以便在后续步骤中进行功能化时，这种相互作用类型能降至最低。文中给出了示例。需要洗脱液梯度和/或洗脱液浓度阶跃变化的方法特别适用于此柱，因为在梯度条件下背景电导率几乎保持不变。
