Kostál Vladimír, Berková Petra, Simek Petr
Institute of Entomology, Academy of Sciences of the Czech Republic, Ceské Budejovice, Branisovská 31, 370 05, Ceské Budejovice, Czech Republic.
Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul;135(3):407-19. doi: 10.1016/s1096-4959(03)00117-9.
The composition of molecular species of phosphatidylethanolamines (PEs) and phosphatidylcholines (PCs) was analysed in fat body and muscle tissues of Chymomyza costata larvae of different physiological states that markedly differed in their level of freeze-tolerance. Actively moving and feeding 3rd instar larvae had low (zero) capacity of freeze-tolerance and similar phospholipid (PL) compositions irrespective of their developmental destiny (non-diapause vs. diapause). Extensive remodelling of PL composition was found in these larvae in response to: (a) chilling of non-diapause larvae at 5 degrees C for 1 month; (b) developmental transition to diapause; and (c) chilling of diapause larvae. Transition to diapause and chilling led to an increase in freeze-tolerance. The increase in molar proportion of molecular species containing palmitic/linoleic (16:0/18:2) fatty acyls (FAs) esterified to sn-1/sn-2 positions of glycerol was the most prominent change, which was tightly statistically correlated with increasing freeze-tolerance. The increase of PLs with combination of 16:0/18:2 FAs was registered consistently in PEs and PCs in fat body and muscle tissues in response to chilling and to diapause onset. This increase was countered by a decreases of various molecular species, depending on tissue and lipid class. Most decreasing species shared one common theme: they had a saturated FA (palmityl, margaryl, stearyl) esterified at sn-1 position and a monounsaturate (palmitoleyl, oleyl) esterified at sn-2 position of glycerol. Possible adaptive meaning of PL molecular species remodelling is discussed.
对不同生理状态、耐寒能力显著不同的科斯塔氏酪蝇幼虫脂肪体和肌肉组织中的磷脂酰乙醇胺（PEs）和磷脂酰胆碱（PCs）分子种类组成进行了分析。活跃移动和取食的3龄幼虫耐寒能力低（为零），且无论其发育命运（非滞育与滞育）如何，磷脂（PL）组成相似。在这些幼虫中发现了PL组成的广泛重塑，这是对以下情况的反应：（a）将非滞育幼虫在5℃下冷藏1个月；（b）发育转变为滞育；以及（c）将滞育幼虫冷藏。向滞育的转变和冷藏导致耐寒能力增加。含有棕榈酸/亚油酸（16:0/18:2）脂肪酰基（FAs）且酯化为甘油sn-1/sn-2位置的分子种类的摩尔比例增加是最显著的变化，这与耐寒能力的增加在统计学上紧密相关。在脂肪体和肌肉组织的PEs和PCs中，随着冷藏和滞育开始，始终记录到含有16:0/18:2 FAs组合的PLs增加。这种增加被各种分子种类的减少所抵消，这取决于组织和脂质类别。大多数减少的种类有一个共同特点：它们在甘油的sn-1位置有一个饱和脂肪酸（棕榈酰、十七烷酰、硬脂酰）酯化，在sn-2位置有一个单不饱和脂肪酸（棕榈油酰、油酰）酯化。讨论了PL分子种类重塑可能的适应性意义。
