Rohde D, Niedermeyer H, Fellbaum C, Hansmann M L, Busch R, Lauder I, Höfler H, Fischer R
Institut für Pathologie, Universität Köln.
Verh Dtsch Ges Pathol. 1992;76:177-80.
Studies demonstrating Epstein-Barr virus (EBV) DNA in Hodgkin's disease (HD) provide little information about the EBV-status in the lymphocyte predominant subtype (nodular paragranuloma) which is due to the small number of cases investigated. Therefore we studied 99 typical cases of nodular paragranuloma for the presence of EBV-DNA using the polymerase chain reaction (PCR) technique. Genomic DNA was amplifiable in 71 cases; 29 cases (= 40%) were positive for EBV-DNA (EBNA-1). In situ hybridization revealed EBV-RNA (EBER-1) in L&H cells and in a few lymphocytes. PCR results were correlated to clinical follow-up data and did not show any statistically significant relationship between EBV positivity and survival of the patients.
有关霍奇金淋巴瘤（HD）中存在爱泼斯坦-巴尔病毒（EBV）DNA的研究，由于所调查的病例数量较少，几乎没有提供关于淋巴细胞为主型亚型（结节性副肉芽肿）中EBV状态的信息。因此，我们使用聚合酶链反应（PCR）技术，对99例典型的结节性副肉芽肿病例进行了EBV-DNA检测。71例病例的基因组DNA可扩增；29例（=40%）EBV-DNA（EBNA-1）呈阳性。原位杂交显示L&H细胞和少数淋巴细胞中有EBV-RNA（EBER-1）。PCR结果与临床随访数据相关，未显示EBV阳性与患者生存之间存在任何统计学上的显著关系。
