Anagnostopoulos I, Hummel M, Finn T, Tiemann M, Dimmler C, Korbjuhn P, Dallenbach F, Niedobitek G, Gatter K, Parwaresch M R
Institut für Pathologie, Klinikum Steglitz der FU Berlin.
Verh Dtsch Ges Pathol. 1992;76:202-6.
32 cases of T cell lymphoma of angioimmunoblastic lymphadenopathy type (AILD-TCL) were investigated for their association with Epstein-Barr virus (EBV). Polymerase chain reaction (PCR) for detection of EBV-DNA and in situ hybridization for EBV-encoded small nuclear RNAs (EBER) produced almost identical results, showing that all but one of AILD-TCL cases contained EBV genomes. Three distinctive patterns of EBV infection were observed after immunophenotypical characterization of EBER positive cells: 1. predominant infection of B-immunoblasts (26% of the cases), 2. predominant infection of neoplastic T cells (42% of the cases) and 3. infection of few small lymphocytes (32% of the cases). EBV-encoded latent membrane protein was frequently detectable in cases exhibiting patterns 1 and 2. These findings suggest that, in AILD-TCL patients B cells and especially T cells are highly susceptible to a persistent EBV infection which may lead to a growth advantage of infected cells.
对32例血管免疫母细胞性淋巴结病样T细胞淋巴瘤（AILD-TCL）患者进行了与爱泼斯坦-巴尔病毒（EBV）相关性的研究。检测EBV-DNA的聚合酶链反应（PCR）和检测EBV编码小核RNA（EBER）的原位杂交结果几乎相同，显示除1例AILD-TCL病例外，其余病例均含有EBV基因组。对EBER阳性细胞进行免疫表型特征分析后，观察到3种不同的EBV感染模式：1. B免疫母细胞为主的感染（26%的病例），2. 肿瘤性T细胞为主的感染（42%的病例），3. 少数小淋巴细胞的感染（32%的病例）。在表现出模式1和模式2的病例中，经常可检测到EBV编码的潜伏膜蛋白。这些发现表明，在AILD-TCL患者中，B细胞尤其是T细胞对持续性EBV感染高度易感，这可能导致受感染细胞的生长优势。
