Gundogan Gul İpek
Department of Histology and Embryology, Faculty of Medicine, Istanbul Yeni Yuzyil University, Maltepe Mahallesi, Yılanlı Ayazma Caddesi, No: 26, Cevizlibağ, Zeytinburnu, 34010, İstanbul, Turkey.
Cytotechnology. 2020 Oct 21;72(6):847-54. doi: 10.1007/s10616-020-00428-1.
Only a limited number of techniques are available for assessing the effect of different coating materials on cell adherence to screws. In this study, we describe a simple and inexpensive method for evaluation of cell adhesion on irregular surfaces such as the surgical or implant screws. For this purpose, we prepared semi-submerged screws in the petri dishes using agar. Using BSA- or HA-coated screws, we tested whether BSA or HA could improve cell adherence when used as coating materials. Agar-coated screws were used as internal control. Then the "ratio of cell adherence" was calculated by subtracting the reference RCA value obtained from the agar coated screws (internal control). When compared to that of the non-coated screws both the HA- and BSA-coating improved cell adherence on the screws by 2.34 and 2.72 fold respectively. Similarly, MTT assay data revealed that the metabolic capacities of cells on HA- or BSA-coated screws were improved by 2.36 and 2.86 fold respectively. These findings suggest that this protocol can be used for comparing the ability of cells to attach on irregular surfaces such as dental or orthopedic screws and assessing their viability.
目前仅有有限的几种技术可用于评估不同涂层材料对细胞在螺钉上黏附的影响。在本研究中，我们描述了一种简单且经济的方法，用于评估细胞在诸如外科手术或植入用螺钉等不规则表面上的黏附情况。为此，我们使用琼脂在培养皿中制备了半浸没的螺钉。使用牛血清白蛋白（BSA）或透明质酸（HA）涂层的螺钉，我们测试了BSA或HA作为涂层材料时是否能改善细胞黏附。琼脂涂层的螺钉用作内部对照。然后通过减去从琼脂涂层螺钉（内部对照）获得的参考相对细胞黏附率（RCA）值来计算“细胞黏附率”。与未涂层的螺钉相比，HA涂层和BSA涂层分别使细胞在螺钉上的黏附提高了2.34倍和2.72倍。同样，MTT检测数据显示，在HA涂层或BSA涂层螺钉上的细胞代谢能力分别提高了2.36倍和2.86倍。这些发现表明，该方案可用于比较细胞在诸如牙科或骨科螺钉等不规则表面上的附着能力，并评估其活力。
