利用携带偶氮苯的修饰DNA对RNA聚合酶反应进行光调节。

Photo-regulation of RNA polymerase reaction by use of modified DNA carrying an azobenzene.

作者信息

Asanuma H, Tamaru D, Yoshida T, Yamazawa A, Mitsui T, Okuni T, Kimoto M, Hirao I, Yokoyama S, Komiyama M

机构信息

Research Center for Advanced Science and Technology, Univ. of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Res Suppl. 2001(1):55-6. doi: 10.1093/nass/1.1.55.

DOI:10.1093/nass/1.1.55

PMID:12836261

Abstract

Transcription reaction by T7-RNA polymerase was photo-regulated on the basis of two strategies as depicted in Scheme 1. It was found that incorporation reaction of azobenzene-tethered uridine triphosphate proceeded only when azobenzene took trans-form (Scheme 1(A)). On the other hand, the transcription was more efficient when the azobenzene moiety, tethered to the non-template strand of the promoter DNA, was in its cis-form under UV irradiation (Scheme 1(B)). Thus, azobenzene-tethered DNAs are promising for the photo-regulation of gene-expression.

摘要

基于方案1中所描述的两种策略，T7 - RNA聚合酶的转录反应受到光调控。结果发现，只有当偶氮苯处于反式构象时，偶氮苯连接的三磷酸尿苷的掺入反应才会进行（方案1(A)）。另一方面，当连接到启动子DNA非模板链上的偶氮苯部分在紫外线照射下处于顺式构象时，转录效率更高（方案1(B)）。因此，偶氮苯连接的DNA有望用于基因表达的光调控。