Photo-regulation of transcription by RNA polymerase with azobenzene-tethered promoter.

作者信息

Asanuma Hiroyuki, Liu Mingzhe, Tamaru Daisuke, Liang Xingguo, Komiyama Makoto

机构信息

Research Center of Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):75-6. doi: 10.1093/nass/2.1.75.

DOI:10.1093/nass/2.1.75

PMID:12903112

Abstract

Photo-regulation of transcription reaction by T7-RNA polymerase was investigated by introducing an azobenzene residue into the non-template strand of T7-promoter. It was found that transcription was strongly interfered under dark or visible light irradiation, whereas it efficiently proceeded after UV irradiation. With this photo-responsive promoter, on-off switching of transcription by light irradiation was achieved. This strategy was also effective for the photo-regulation of SP6-RNA polymerase.

摘要