Liu Mingzhe, Komiyama Makoto, Asanuma Hiroyuki
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):283-4. doi: 10.1093/nass/49.1.283.
Efficient photoregulation of transcription by T7 RNA polymerase (RNAP) has been achieved with modified T7-promoter involving two azobenzene moieties at RNAP recognition region and TATA region simultaneously. Transcription proceeded 7.5-fold faster after UV irradiation than that under dark condition. Kinetic parameters (kcat, Km) for the transcription revealed that an azobenzene at each position played a different role and thus synergistic effect of photo-regulation was achieved with the promoter involving two azobenzenes.
通过在T7启动子的RNA聚合酶（RNAP）识别区域和TATA区域同时引入两个偶氮苯基团对其进行修饰，实现了T7 RNA聚合酶（RNAP）对转录的有效光调控。紫外线照射后转录速度比黑暗条件下快7.5倍。转录的动力学参数（kcat、Km）表明，每个位置的偶氮苯发挥不同作用，因此含有两个偶氮苯的启动子实现了光调控的协同效应。
