末端标记RNA序列的合成：RNA-DNA双链体的荧光与杂交研究
Synthesis of terminally labelled RNA sequences: fluorescence and hybridisation study of RNA-DNA duplexes.
作者信息
Misra A, Tripathi S, Misra K
机构信息
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211 002, India.
出版信息
Nucleic Acids Res Suppl. 2001(1):95-6. doi: 10.1093/nass/1.1.95.
PMID:12836281
Abstract
A stable and suitably labelled DNA-RNA hybrid, resistant to RNAse degradation has been prepared by hybridising the RNA sequence carrying methoxy group at 2'-position of ribose moiety in adenosines and labelled with dansyl group attached via a linker at the terminal uridyl unit. Study may be useful in sequencing and targeting specific viral sequences.
摘要
通过将在腺苷核糖部分2'-位带有甲氧基且在末端尿苷单元通过连接子连接有丹磺酰基团标记的RNA序列进行杂交，制备了一种稳定且带有适当标记、抗核糖核酸酶降解的DNA-RNA杂交体。该研究可能对特定病毒序列的测序和靶向有用。
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