酵母非编码RNA加工的全景图。

A panoramic view of yeast noncoding RNA processing.

作者信息

Peng Wen Tao, Robinson Mark D, Mnaimneh Sanie, Krogan Nevan J, Cagney Gerard, Morris Quaid, Davierwala Armaity P, Grigull Jörg, Yang Xueqi, Zhang Wen, Mitsakakis Nicholas, Ryan Owen W, Datta Nira, Jojic Vladimir, Pal Chris, Canadien Veronica, Richards Dawn, Beattie Bryan, Wu Lani F, Altschuler Steven J, Roweis Sam, Frey Brendan J, Emili Andrew, Greenblatt Jack F, Hughes Timothy R

机构信息

Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada.

出版信息

Cell. 2003 Jun 27;113(7):919-33. doi: 10.1016/s0092-8674(03)00466-5.

DOI:10.1016/s0092-8674(03)00466-5

PMID:12837249

Abstract

Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA.

摘要

利用公开可用的酵母功能基因组学和蛋白质组学数据进行的预测分析表明，参与核糖核蛋白生物合成的蛋白质可能比目前已知的要多得多。我们使用了一个监测非编码RNA丰度和加工过程的微阵列，分析了468个携带蛋白质编码基因突变的酵母菌株，其中大多数以前并未与RNA或RNP合成相关联。许多在未表征基因中发生突变的菌株显示出异常的非编码RNA谱。通过进一步实验验证了10个参与非编码RNA生物合成的因子，包括20S前体rRNA加工所需的一种蛋白质（Tsr2p）、与核外泌体相关的一种蛋白质（Lrp1p）以及盒C/D snoRNA积累所需的一个因子（Bcd1p）。这些数据展示了酵母非编码RNA加工的全局视图，并证实许多目前未表征的酵母蛋白质参与了非编码RNA的生物合成。