Blaurock B, Hippeli S, Metz N, Elstner E F
Institut für Botanik und Mikrobiologie, Biochemisches Labor, Technische Universität München, Federal Republic of Germany.
Arch Toxicol. 1992;66(10):681-7. doi: 10.1007/BF01972618.
Aqueous solutions of engine exhaust condensation products were derived from cars powered by diesel or four-stroke gasoline engines (with and without three-way catalytic converter). The cars were operated on a static test platform. Samples of the different exhaust solutions accumulated in a Grimmer-type distillation trap (VDI 3872) during standard test programs (Federal Test Procedure) were incubated with important biomolecules. As indicators of reactive oxygen species or oxidative destruction, ascorbic acid, cysteine, glutathione, serum albumin, the enzymes glycerinaldehyde phosphate dehydrogenase and xanthine oxidase, and the oxygen free-radical indicator keto-methylthiobutyrate were used. During and after the incubations, oxygen activation (consumption) and oxidative destruction were determined. Comparison of the oxidative activities of the different types of exhaust condensates clearly showed that the exhaust condensate derived from the four-stroke car equipped with a three-way catalytic converter exhibited by far the lowest oxidative and destructive power.
发动机尾气冷凝产物的水溶液来自以柴油或四冲程汽油发动机驱动的汽车（有或没有三元催化转化器）。这些汽车在静态测试平台上运行。在标准测试程序（联邦测试程序）期间，收集在格林默式蒸馏阱（VDI 3872）中的不同尾气溶液样本与重要生物分子一起孵育。使用抗坏血酸、半胱氨酸、谷胱甘肽、血清白蛋白、甘油醛磷酸脱氢酶和黄嘌呤氧化酶等酶以及氧自由基指示剂酮基甲基硫代丁酸酯作为活性氧或氧化破坏的指标。在孵育期间和之后，测定氧活化（消耗）和氧化破坏情况。不同类型尾气冷凝物氧化活性的比较清楚地表明，配备三元催化转化器的四冲程汽车产生的尾气冷凝物氧化和破坏能力最低。
