Domoto T, Oki M, Kotoh T, Nakamura T
Department of Anatomy, Shimane Medical University, Izumo, Japan.
Clin Auton Res. 1992 Dec;2(6):403-7. doi: 10.1007/BF01831399.
The distribution of nerve fibres immunoreactive for vasoactive intestinal polypeptide (VIP), substance P (SP), methionine-enkephalin (ENK), calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) within the circular muscle layer was examined histochemically in the human pylorus, adjacent antrum and duodenum. Longitudinal cryostat sections of the pyloric and surrounding regions were stained by an indirect immunofluorescence method, and the total length of each type of peptide-containing fibre per unit sectional area (micron/mm2) was measured using an image-analysing system. The narrow region of the circular muscle layer bordering the submucosa in the pylorus contained a rich supply of VIP, SP, ENK and CGRP immunoreactive fibres; VIP fibres were most prominent with less SP and ENK fibres and moderate amounts of CGRP. These peptide-containing nerve fibres were more dense than in the pyloric circular muscle, the longitudinal muscle layer and also the adjacent muscle layer. NPY-immunoreactive fibres were sparsely distributed throughout the pyloric region. These results suggest that the inner edge of the circular muscle, lying adjacent to the submucosa and densely innervated with peptide-containing fibres, may be a characteristic feature of the human pyloric sphincter.
采用组织化学方法，对人幽门、毗邻胃窦及十二指肠环形肌层内血管活性肠肽（VIP）、P物质（SP）、甲硫氨酸脑啡肽（ENK）、降钙素基因相关肽（CGRP）和神经肽Y（NPY）免疫反应性神经纤维的分布进行了研究。幽门及周围区域的连续冰冻切片采用间接免疫荧光法染色，使用图像分析系统测量单位截面积（微米/平方毫米）内每种含肽纤维的总长度。幽门黏膜下层附近的环形肌层狭窄区域含有丰富的VIP、SP、ENK和CGRP免疫反应性纤维；VIP纤维最为突出，SP和ENK纤维较少，CGRP含量适中。这些含肽神经纤维比幽门环形肌、纵行肌层以及相邻肌层中的更为密集。NPY免疫反应性纤维稀疏分布于整个幽门区域。这些结果表明，环形肌的内边缘毗邻黏膜下层，且富含含肽纤维，这可能是人类幽门括约肌的一个特征。
