母体β-连环蛋白在非洲爪蟾早期中胚层诱导中的作用。
A role for maternal beta-catenin in early mesoderm induction in Xenopus.
作者信息
Schohl Anne, Fagotto François
机构信息
Department of Cell Biology, Max-Planck Institute for Developmental Biology, Tübingen, Germany.
出版信息
EMBO J. 2003 Jul 1;22(13):3303-13. doi: 10.1093/emboj/cdg328.
Abstract
Mesoderm formation results from an inducing process that requires maternal and zygotic FGF/MAPK and TGFbeta activities, while maternal activation of the Wnt/beta-catenin pathway determines the anterior-dorsal axis. Here, we show a new role of Wnt/beta-catenin signaling in mesoderm induction. We find that maternal beta-catenin signaling is not only active dorsally but also all around the equatorial region, coinciding with the prospective mesoderm. Maternal beta-catenin function is required both for expression of dorsal genes and for activation of MAPK and the mesodermal markers Xbra and eomesodermin. beta-catenin acts in a non- cell-autonomous manner upstream of zygotic FGF and nodal signals. The Wnt/beta-catenin activity in the equatorial region of the early embryo is the first example of a maternally provided mesoderm inducer restricted to the prospective mesoderm.
摘要
中胚层的形成源于一个诱导过程，该过程需要母体和合子的FGF/MAPK以及TGFβ活性，而Wnt/β-连环蛋白信号通路的母体激活决定了前后背轴。在此，我们展示了Wnt/β-连环蛋白信号在中胚层诱导中的新作用。我们发现母体β-连环蛋白信号不仅在背部活跃，而且在整个赤道区域都活跃，这与预期的中胚层区域一致。母体β-连环蛋白功能对于背侧基因的表达以及MAPK和中胚层标记物Xbra和中胚层决定因子的激活都是必需的。β-连环蛋白在合子FGF和节点信号的上游以非细胞自主的方式起作用。早期胚胎赤道区域的Wnt/β-连环蛋白活性是母体提供的仅限于预期中胚层的中胚层诱导物的首个例子。
相似文献
1
A role for maternal beta-catenin in early mesoderm induction in Xenopus.母体β-连环蛋白在非洲爪蟾早期中胚层诱导中的作用。
EMBO J. 2003 Jul 1;22(13):3303-13. doi: 10.1093/emboj/cdg328.
2
The Wnt/beta-catenin pathway posteriorizes neural tissue in Xenopus by an indirect mechanism requiring FGF signalling.在非洲爪蟾中，Wnt/β-连环蛋白信号通路通过一种需要FGF信号传导的间接机制使神经组织向尾侧分化。
Dev Biol. 2001 Nov 1;239(1):148-60. doi: 10.1006/dbio.2001.0431.
3
Nodal signaling in Xenopus gastrulae is cell-autonomous and patterned by beta-catenin.非洲爪蟾原肠胚中的节点信号是细胞自主的，并由β-连环蛋白形成模式。
Dev Biol. 2003 Jan 1;253(1):125-38. doi: 10.1006/dbio.2002.0867.
4
Zygotic Wnt/beta-catenin signaling preferentially regulates the expression of Myf5 gene in the mesoderm of Xenopus.合子型Wnt/β-连环蛋白信号通路优先调控非洲爪蟾中胚层中Myf5基因的表达。
Dev Biol. 2002 May 1;245(1):124-35. doi: 10.1006/dbio.2002.0633.
5
The maternal Xenopus beta-catenin signaling pathway, activated by frizzled homologs, induces goosecoid in a cell non-autonomous manner.非洲爪蟾母体的β-连环蛋白信号通路由卷曲同源物激活，以非细胞自主方式诱导鹅膏蕈氨酸。
Dev Growth Differ. 2000 Aug;42(4):347-57. doi: 10.1046/j.1440-169x.2000.00517.x.
6
Anterior endomesoderm specification in Xenopus by Wnt/beta-catenin and TGF-beta signalling pathways.非洲爪蟾中Wnt/β-连环蛋白和TGF-β信号通路对前端内胚层的特化作用
Dev Biol. 1999 May 15;209(2):282-97. doi: 10.1006/dbio.1999.9257.
7
Neural induction in the absence of mesoderm: beta-catenin-dependent expression of secreted BMP antagonists at the blastula stage in Xenopus.在没有中胚层的情况下进行神经诱导：非洲爪蟾囊胚期分泌型骨形态发生蛋白拮抗剂的β-连环蛋白依赖性表达
Dev Biol. 2001 Jun 1;234(1):161-73. doi: 10.1006/dbio.2001.0258.
8
Cortical rotation is required for the correct spatial expression of nr3, sia and gsc in Xenopus embryos.非洲爪蟾胚胎中nr3、sia和gsc的正确空间表达需要皮层旋转。
Int J Dev Biol. 1997 Oct;41(5):741-5.
9
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.钙黏蛋白的过表达和β-连环蛋白的低表达会抑制非洲爪蟾早期胚胎中背侧中胚层的诱导。
Cell. 1994 Dec 2;79(5):791-803. doi: 10.1016/0092-8674(94)90069-8.
10
xBtg-x regulates Wnt/beta-Catenin signaling during early Xenopus development.在非洲爪蟾早期发育过程中，xBtg - x调节Wnt/β - 连环蛋白信号通路。
Dev Biol. 2005 Jul 1;283(1):17-28. doi: 10.1016/j.ydbio.2005.03.033.
引用本文的文献
1
c-JUN: a chromatin repressor that limits mesoderm differentiation in human pluripotent stem cells.c-JUN：一种限制人类多能干细胞中胚层分化的染色质阻遏物。
Nucleic Acids Res. 2025 Jan 24;53(3). doi: 10.1093/nar/gkaf001.
2
Differentiation of Pluripotent Stem Cells for Disease Modeling: Learning from Heart Development.用于疾病建模的多能干细胞分化：从心脏发育中汲取经验。
Pharmaceuticals (Basel). 2024 Mar 5;17(3):337. doi: 10.3390/ph17030337.
3
Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells by WNT Switch Method.利用 WNT 转换方法从小鼠胚胎干细胞中诱导心肌细胞分化。
Cells. 2024 Jan 11;13(2):132. doi: 10.3390/cells13020132.
4
Combined synchronization and superovulation treatments negatively impact embryo viability possibly by the downregulation of WNT/β-catenin and Notch signaling genes in the porcine endometrium.联合同步发情和超数排卵处理可能通过下调猪子宫内膜中的 WNT/β-连环蛋白和 Notch 信号基因来降低胚胎活力。
J Anim Sci. 2022 Nov 1;100(11). doi: 10.1093/jas/skac315.
5
The Organizer and Its Signaling in Embryonic Development.胚胎发育中的组织者及其信号传导
J Dev Biol. 2021 Nov 1;9(4):47. doi: 10.3390/jdb9040047.
6
Repression of Inappropriate Gene Expression in the Vertebrate Embryonic Ectoderm.脊椎动物胚胎外胚层中异常基因表达的抑制。
Genes (Basel). 2019 Nov 6;10(11):895. doi: 10.3390/genes10110895.
7
TGF-β Family Signaling in Early Vertebrate Development.TGF-β 家族信号在早期脊椎动物发育中的作用。
Cold Spring Harb Perspect Biol. 2018 Jun 1;10(6):a033274. doi: 10.1101/cshperspect.a033274.
8
A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs.一个控制非洲爪蟾早期中内胚层形成的基因调控程序：网络保守性与基序
Semin Cell Dev Biol. 2017 Jun;66:12-24. doi: 10.1016/j.semcdb.2017.03.003. Epub 2017 Mar 22.
9
Ubiquitin C-terminal hydrolase37 regulates Tcf7 DNA binding for the activation of Wnt signalling.泛素 C 端水解酶 37 调节 Tcf7 的 DNA 结合以激活 Wnt 信号通路。
Sci Rep. 2017 Feb 15;7:42590. doi: 10.1038/srep42590.
10
CFTR-β-catenin interaction regulates mouse embryonic stem cell differentiation and embryonic development.CFTR与β-连环蛋白的相互作用调节小鼠胚胎干细胞分化和胚胎发育。
Cell Death Differ. 2017 Jan;24(1):98-110. doi: 10.1038/cdd.2016.118. Epub 2016 Nov 11.
本文引用的文献
1
The formation of the mesoderm in urodelean amphibians : I. Induction by the endoderm.有尾两栖动物中胚层的形成：I. 内胚层诱导
Wilhelm Roux Arch Entwickl Mech Org. 1969 Dec;162(4):341-373. doi: 10.1007/BF00578701.
2
Zygotic Wnt activity is required for Brachyury expression in the early Xenopus laevis embryo.合子Wnt活性是非洲爪蟾早期胚胎中Brachyury基因表达所必需的。
Dev Biol. 2002 Oct 1;250(1):112-27. doi: 10.1006/dbio.2002.0786.
3
Beta-catenin, MAPK and Smad signaling during early Xenopus development.非洲爪蟾早期发育过程中的β-连环蛋白、丝裂原活化蛋白激酶和Smad信号传导
Development. 2002 Jan;129(1):37-52. doi: 10.1242/dev.129.1.37.
4
Axis induction by wnt signaling: Target promoter responsiveness regulates competence.Wnt信号通路介导的轴诱导：靶启动子反应性调节感受态。
Dev Biol. 2001 Jun 1;234(1):42-54. doi: 10.1006/dbio.2001.0253.
5
Maternal VegT is the initiator of a molecular network specifying endoderm in Xenopus laevis.母体维甲酸应答蛋白（VegT）是非洲爪蟾中决定内胚层的分子网络的启动因子。
Development. 2001 Jan;128(2):167-80. doi: 10.1242/dev.128.2.167.
6
Expression of activated MAP kinase in Xenopus laevis embryos: evaluating the roles of FGF and other signaling pathways in early induction and patterning.非洲爪蟾胚胎中活化丝裂原活化蛋白激酶的表达：评估成纤维细胞生长因子及其他信号通路在早期诱导和模式形成中的作用
Dev Biol. 2000 Dec 1;228(1):41-56. doi: 10.1006/dbio.2000.9917.
7
Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center.两个新的与节点相关的基因在非洲爪蟾Nieuwkoop中心引发早期诱导事件。
Development. 2000 Dec;127(24):5319-29. doi: 10.1242/dev.127.24.5319.
8
Vertebrate mesendoderm induction and patterning.脊椎动物中胚层内胚层的诱导与模式形成。
Curr Opin Genet Dev. 2000 Aug;10(4):350-6. doi: 10.1016/s0959-437x(00)00095-2.
9
Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development.非洲爪蟾早期发育过程中转化生长因子β超家族信号的内源性模式。
Development. 2000 Jul;127(13):2917-31. doi: 10.1242/dev.127.13.2917.
10
The putative wnt receptor Xenopus frizzled-7 functions upstream of beta-catenin in vertebrate dorsoventral mesoderm patterning.推测的Wnt受体非洲爪蟾卷曲蛋白-7在脊椎动物背腹侧中胚层模式形成中β-连环蛋白的上游发挥作用。
Development. 2000 May;127(9):1981-90. doi: 10.1242/dev.127.9.1981.
Zotero 插件