Reuter D, Polywka S, Iske L, Feucht H H, Laufs R
Institut für Medizinische Mikrobiologie und Immunologie, Hamburg, Germany.
Infection. 1992 Nov-Dec;20(6):320-3. doi: 10.1007/BF01710675.
To determine whether the persistent presence of antibodies to recombinant antigens of the hepatitis C virus (HCV) corresponds to the presence of hepatitis C virus RNA in the same serum, 85 anti-HCV positive patients were studied by the polymerase chain reaction (PCR). The focus of the research was on patients with chronic hepatitis. Eighty- three patients were found to be positive by PCR; only two were negative. In addition, liver biopsies taken from seven patients positive for anti-HCV were shown to contain HCV-specific RNA. Sera collected from three patients suspected to have NANB hepatitis on the basis of clinical symptoms were negative both for HCV antibodies and HCV RNA. The correlation between HCV antibody positivity and detection of HCV RNA was 97.6%.
为了确定丙型肝炎病毒（HCV）重组抗原抗体的持续存在是否与同一血清中丙型肝炎病毒RNA的存在相对应，通过聚合酶链反应（PCR）对85例抗-HCV阳性患者进行了研究。研究重点是慢性肝炎患者。83例患者经PCR检测呈阳性；仅2例为阴性。此外，对7例抗-HCV阳性患者进行的肝活检显示含有HCV特异性RNA。根据临床症状怀疑患有非甲非乙型肝炎的3例患者的血清，HCV抗体和HCV RNA均为阴性。HCV抗体阳性与HCV RNA检测之间的相关性为97.6%。
